~125Ⅰ粒子对Hela细胞杀伤作用的实验研究  

Experimental study on the killing effect of ~125I seeds to Hela cells

作　　者：苏成海[1] 法逸华[1] 许玉杰[2] 范我[2] 

机构地区：[1]苏州大学附属第一医院核医学科,215006 [2]苏州大学放射医学与公共卫生学院

出　　处：《中华核医学杂志》2005年第6期372-374,i0001,共4页Chinese Journal of Nuclear Medicine

摘　　要：目的观察体外 Hela 细胞在^(125)Ⅰ粒子照射下细胞凋亡和增殖的规律,探讨^(125)Ⅰ粒子对肿瘤的杀伤作用。方法用^(125)Ⅰ粒子照射 Hela 细胞,用不同的方法观察不同剂量和时间点细胞的增殖反应及形态变化。结果受照射细胞发生凋亡,于Ⅱb期出现凋亡小体;细胞凋亡率随累积剂量的增加而增加,于5 Gy 时出现凋亡率峰值;细胞克隆形成率显著下降,与凋亡形成呈反向关系。并有反剂量率效应。结论 ^(125)Ⅰ粒子对肿瘤的治疗作用可能为早期抑制肿瘤细胞增殖,晚期使肿瘤细胞大量凋亡。Objective To investigate the rules of apoptosis and proliferation of Hela cells in vitro irradiated with ^125I seeds and the killing effect to tumor cells. Methods The proliferation reaction and morphological change of Hela cells with low energy γ ray ^125I irradiation in different dose and duration were observed. Results The apoptosis segments of Hela cells irradiated with ^125I appeared was on stage Ⅱb. However, significant increase in the cell apoptosis ratio related to the dose accumulation and apoptosis reached peak level at a total dose of 5 Gy. Meanwhile, the results of the cell clones generally correlated negatively with apoptosis and demonstrated a significant reduction. A reverve dose rate effect was also found. Conclusion The therapeutic effect of ^125I seeds irradiation probably may be the inhibition of cell proliferation in early stage, and apoptosis of large amount of tumor cells in later stage.

关 键 词：放射疗法 碘放射性同位素 HELA细胞 肿瘤细胞 培养的 

分 类 号：R737.33[医药卫生—肿瘤]