基质金属蛋白酶-2在实验性近视眼鸡巩膜成纤维细胞的表达  

MMP-2 expression in scleral fibroblasts of experimental myopia chick

作　　者：蒋莉[1] 刘双珍[1] 王剑锋[1] 

机构地区：[1]中南大学湘雅医院眼科,湖南省长沙市410008

出　　处：《眼科新进展》2005年第6期516-518,共3页Recent Advances in Ophthalmology

摘　　要：目的研究基质金属蛋白酶-2(matrix metalloproteinases-2,MMP-2)在形觉剥夺性近视眼(formdeprivation myopia,FDM)鸡巩膜成纤维细胞的表达。方法20只1d龄来亨雏鸡以半透明眼罩遮盖右眼14d制备FDM动物模型,随机取10只FDM眼去除遮盖7d作为恢复组,均以对侧未遮盖眼作为对照组。将各组小鸡眼后极部巩膜成纤维细胞作体外培养并传2代,行光镜与透射电镜形态学观察,并采用SABC免疫细胞化学染色法检测MMP-2的表达,进行计算机图像分析及统计学检验。结果培养的正常鸡巩膜成纤维细胞胞浆表达MMP-2,阳性灰度值为192·2319±1·2521。FDM组细胞MMP-2染色阳性灰度值为168·1730±5·0039,较对照组MMP-2表达明显增高(P<0·01)。恢复组阳性灰度值为180·4001±2·3522,其MMP-2表达较FDM组有所回降(P<0·01),但与对照组相比仍有升高,差异有显著性(P<0·01)。结论MMP-2参与形觉剥夺性近视眼的发生发展与恢复的过程,在近视发生机制中起重要作用。Objective To investigate the expression of matrix metalloproteinases-2 （MMP-2） in chick posterior scleral fibroblasts （CPSFs） of experimental myopia. Methods The right eyes of twenty 1-day-old leghorn chicks were closed with a plastic goggle for 14 days to induce form deprivation myopia （FDM）, with the left eye as control. Ten FDM eyes＇ goggles were randomly removed for 7 days as the recovery group. CPSFs of each group were cultured and passaged 2 generations in vitro. MMP-2 expression in cultured CPSFs was evaluated by inununocytochemistry method and light microscope and transmission electron microscope. The results were analyzed by computer image-analysis system and statistical test. Results The cultured CPSFs expressed MMP-2. In comparison with the control group, the MMP-2 expression of the FDM group were much higher. The MMP-2 expression of recovery group were lower than the FDM group while higher than that of the control group.Conclusion MMP-2 could take part in the occurrence,development and recovery process of the FDM and seemed to play an important role in the mechanism of myopia.

关 键 词：形觉剥夺性近视眼 成纤维细胞 基质金属蛋白酶-2 

分 类 号：R778.1[医药卫生—眼科]