急性和慢性束缚应激对大鼠内脏敏感性的影响  

Effects of acute and chronic restraint stress on visceral sensitivity in rats

作　　者：孙燕[1] 柳锋霖[1] 钱伟[1] 侯晓华[1] 

机构地区：[1]华中科技大学附属协和医院消化内科,武汉430030

出　　处：《胃肠病学和肝病学杂志》2005年第6期634-636,共3页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的了解急性和慢性束缚应激对大鼠内脏敏感性的影响和持续时间。方法成年SD大鼠随机分为3组:对照组(没有束缚应激),急性部分束缚应激组(单次2h的束缚应激)和慢性部分束缚应激组(连续束缚应激3天,每天2h),通过腹壁回撤反应(AWR)评分评估应激前后不同时间点大鼠对结直肠扩张(CRD)的内脏敏感性。结果①在扩张压力20mmHg和40mmHg时,急慢性束缚应激组的AWR评分均显著高于应激前基础水平(Od)(P<0.05),但在60,80mmHg压力时各组的AWR评分均无显著差异;②急性束缚组在应激后24h和应激后第7天,AWR评分显著下降(P<0.05);③慢性束缚组在末次应激后24h,AWR评分仍维持增高水平,但在第7天时AWR评分回到基础水平。结论急性和慢性束缚应激都可以导致大鼠内脏敏感性增高,但其作用可能是短暂的。Objective The aim of this study was to investigate the short-and long-term effects of acute and chronic partial restraint stress （PRS） on visceral sensitivity to colorectal distention （CRD） in rats. Methods Twenty one Adult male Spragne-Dawlcy （SD） rats were studied in three groups including rats without PRS, with acute PRS （A-PRS） for single 2 hours, and with chronic PRS （C-PRS） for 2 hours a day in 3 consecutive days. The abdominal withdrawal reflex （AWR） scores to CRD was assessed before stress, at the end of A-PRS or C-PRS, 24 hours of A-PR.S or C-PRS,and 7 days after the stress. Results （1） The AWR scores of the rats with A-PRS or C-PRS at 20 and 40mmHg immediately after stress significantly higher than those before stress （ P 〈 0.05）. But There was no significant difference for the AWR scores in all the groups at 60 and 80 mmHg. （2） The AWR scores in the A-PRS decreased significantly after 24 hours and seven days after the stress （P 〈 0.05）. （3） The AWR scores in the C-PRS group were higher still after 24 hours of the stress （P 〈 0.05）, but decreased significantly on the 7th day （ P 〈 0.05）. Conclusion Both acute and chronic PRS induce visceral hypersensitivity, but their effects may be transient.

关 键 词：急性束缚应激 慢性束缚应激 内脏敏感性 

分 类 号：R574.62[医药卫生—消化系统]