食管癌组织中P53、P-gp等蛋白的表达  

Expression of six genes in esophageal carcinoma and their clinical significance.

作　　者：邓春美[1] 兰海涛[1] 董丹丹[1] 吴琦[1] 杨红[1] 

机构地区：[1]四川省医学科学院四川省人民医院,四川成都610072

出　　处：《现代肿瘤医学》2006年第1期20-23,共4页Journal of Modern Oncology

基　　金：四川省人民医院科研课题[省医行(2001)062号]

摘　　要：目的检测食管癌组织中P53、多药耐药基因(mu ltidrug resistance gene1,MDR1)及其编码产物P-糖蛋白(p-glycoprote in,P-gp)、肺耐药蛋白(lung resistance prote in,LRP)、谷胱甘肽S-转移酶(glutath ione S-transferase,GST-π)、拓扑异构酶(topoisom eraseⅡ,TopoⅡ)的表达水平,探讨其与临床分期、分化程度、淋巴结转移、肿瘤浸润深度及病理分型的关系。方法采用免疫组织化学技术检测P53、MDR1、LRP、GST-π、TopoⅡ基因的表达水平。结果66例标本中,P53、MDR1、LRP、GST-π、TopoⅡ基因阳性表达率分别为65.2%(43/66)、97.0%(64/66)、84.8%(56/66)、60.6%(40/66)、37.9%(25/66)。食管癌不同组织学分级的TOPOⅡ表达率不同,高分化的表达率高于中低分化者,但不具显著性差异(P=0.05)。LRP的阳性表达率在不同浸润深度之间有显著性差异。P-gp的阳性表达率在溃疡型食管鳞状细胞癌中明显高于其他两种类型,有显著性差异,P=0.003。结论食管癌组织中P53、MDR1、LRP、GST-π、TopoⅡ基因存在高表达;肿瘤组织分化越高,TopoⅡ表达率越高;肿瘤浸润越深,LRP阳性表达率越高。这些检测对临床化疗药物选择可起一定指导作用。Objective To detect the expression of p53、multidrug resistance genel （ mdrl ） gene, glutathione S-transferase （ GST-π ） , lung resistance protein （ LRP ） , topoisomerase Ⅱ （ Topo Ⅱ ） , and to evaluate their relationship with staging and classification of cancer, differentiation degree, lymphatic metastasis, and tumor invasion. Methods In the sixty-six patients diagnosed as esophageal carcinoma, immunohistochemical technique was used to investigate the expression of p53,mdr-1, GST-π, LRP, and Topo Ⅱ. Results The rates of expression of p53, mdr1, LRP, GST-π, and Tope Ⅱ were 65.2 %（43/66）, 97.0%（64/66）, 84.9 %（56/66）, 60.6%（40/66） , and 37.9% （25/66） , respectively. There was different Topo Ⅱ expression with different histological classification of esophageal carcinoma. Tope Ⅱ expression were higher in metastatic group than the non metastatic group, but there was no significant difference. LRP expression had significant differences in different invasion tumor types. Expression of PgP in ulcer esophageal squamous cell carcinoma was obviously higher than the other two types, and there was a significant difference. Conclusion There is intrinsically high expression of p53,mdr1, LRP, GST-π, and Tope Ⅱ in the esophageal carcinoma tissue. The higher differentiation, the higher expression of genes mentioned above; the deeper tumor invasion, the higher expression of LRP. These markers play an important role in choosing clinical chemotherapy drugs.

关 键 词：食管肿瘤 P53 多药耐药基因 P-糖蛋白 肺耐药蛋白 谷胱甘肽转移酶 拓扑异构酶 免疫组织化学 

分 类 号：R735.1[医药卫生—肿瘤]