甲壳低聚糖对α-葡萄糖苷酶活性的影响及降血糖作用  

Effects of chitooligosaccharides on α-glucosidase and its lowering blood-glucose activity

作　　者：曹朝晖[1] 董晓英[1] 方敏[2] 李邦良[1] 

机构地区：[1]南华大学生物化学与分子生物学教研室,湖南衡阳421001 [2]长沙市卫生学校,湖南长沙410100

出　　处：《中国生化药物杂志》2005年第6期327-329,共3页Chinese Journal of Biochemical Pharmaceutics

基　　金：湖南省教育厅课题(No.4-02-JY-02C399)

摘　　要：目的探讨甲壳低聚糖对α-葡萄糖苷酶活性的影响及降糖效果。方法在体外建立标准酶反应体系,分别加入6%甲壳低聚糖50,100,150,200,250,300μl,用4-硝基酚-α-D呋喃葡萄糖苷法测定酶活性;再建立加有6%阿卡波糖200μl的酶反应体系,测定酶活性;以250 mg/(kg.d)剂量灌胃,观察其对实验性糖尿病大鼠空腹及餐后血糖的影响。结果甲壳低聚糖对α-葡萄糖苷酶具有明显抑制作用,抑制效果接近于阿卡波糖;甲壳低聚糖能显著降低糖尿病大鼠空腹及餐后血糖。结论甲壳低聚糖可能具有阿卡波糖样的降血糖作用。Purpose To study the effects of chitooligosaccharides on α-glucosidase activity and hypoglycemic activity in experimental type 2 diabetic rats. Methods An enzyme-inhibitor model was established to observe inhibition of α-glucosidase activity by chitooligosaccharides or acarbose, chitooligosaccharides or acarbose was given to mice at a dose of 250 mg/（ kg·d）（ ig, for 8 weeks）, and the effect on glucose level of diabeticmice was observed. Results Chitoofigosaccharides could obviously inhibit α-glucosidase activity, and decrease the levels of fasting or pasting glucose. Conclusion This finding suggests that the hypoglycemic effect of chitooligosaccharides is similar to that of acarbose.

关 键 词：甲壳低聚糖 Α-葡萄糖苷酶 抑制作用 

分 类 号：Q538[生物学—生物化学] R977.15[医药卫生—药品]