利用RAMP和ISSR标记分析大麦种质资源的遗传多样性  

Genetic Diversity Among Barley Germplasm with Known Origins Based on the RAMP and ISSR Markers

作　　者：侯永翠[1] 颜泽洪[1] 兰秀锦[1] 魏育明[1] 郑有良[2] 

机构地区：[1]四川农业大学小麦研究所,都江堰611830 [2]西南作物基因资源与遗传改良教育部重点实验室,雅安625014

出　　处：《中国农业科学》2005年第12期2555-2565,共11页Scientia Agricultura Sinica

基　　金：国家"863"计划项目(2003AA207100);高等学校全国优秀博士学位论文作者专项资金(200357);长江学者和创新团队发展计划(IRT0453)资项目助

摘　　要：利用RAMP和ISSR两种标记分别对60份大麦种质资源遗传多样性进行了检测。结果发现,两种标记都能揭示材料间较高的遗传多样性,其中ISSR标记多态性又高于RAMP标记。在RAMP分析中,每个引物可扩增出1～10条DNA片段,平均为5.27条;22个RAMP引物共扩增出116条DNA片段,其中98条具有多态性,多态性比率(PPB)为84.48%;平均多态信息量(PIC)为0.277;每个位点有效等位基因数(Ne)为1.602;材料间遗传相似系数GS变化范围为0.551～0.965,平均值为0.830。在ISSR分析中,每个引物可获得1～10条DNA片段,平均为5.95条;19个ISSR引物共扩增出113条DNA片段,其中111条具有多态性,多态性比率(PPB)为98.23%;平均多态信息量(PIC)为0.427;每个位点有效等位基因数(Ne)为2.033;材料间遗传相似系数GS变幅为0.203～0.931,平均达0.676。聚类分析表明,两种标记都能将供试材料完全区分开,聚类结果具有一定的相似性,但也存在明显差异。Mantel检测表明,这两种标记极显著相关(r=0.346,t=2.808)。The genetic diversity and genetic relationships among 60 barley accessions, including 33 Chinese endemic barley landraces, 19 wild barley accessions from Xizang, China, and 8 barley cultivars from other countries, were evaluated by RAMP and ISSR markers. In RAMP analysis, a total of 116 bands were observed in 22 markers, among with 98 bands were polymorphic. Each primer could amplify 1 to 10 polymorphic bands, with an average of 5.27 bands. The percentage of polymorphic bands （PPB）, mean polymorphism information content （PIC） and effective number of alleles （Ne） were 84.48%, 0.277 and 1.602, respectively. Among the 60 barley accessions, the RAMP-based genetic similarity （RAMP-GS） ranged from 0.551 to 0.965, with a mean of 0.830, In ISSR analysis, a total of ll3 bands were detected, among which 111 bands were polymorphic. The number of bands from each ISSR marker ranged from 1 to 10, with an average of 5.95. The PPB, PIC and Ne were 98.23%, 0.427 and 2.033, respectively, The ISSR-derived genetic similarity （ISSR-GS） ranged from 0.203 to 0.931, with a mean of 0.676. The cluster analysis indicated that all the 60 barley accessions could be distinguished by both RAMP and ISSR markers. Mantel test indicated that RAMP and ISSR were significantly associated. These results suggested that high level of genetic diversity among the barley germplasm has been detected by RAMP and ISSR markers, and ISSR was superior to RAMP.

关 键 词：大麦 RAMP ISSR 遗传多样性 遗传关系 聚类分析 

分 类 号：S512.3[农业科学—作物学]