Tropic1808基因作用于PC12细胞的表达谱芯片分析  

Gene Expression Profiles in PC12: Alterations Induced by Tropic1808 Gene Over Expression

作　　者：刘梅[1] 刘炎[2] 林社裕[2] 丁斐[2] 顾晓松[2] 

机构地区：[1]江苏大学生命科学院,镇江212013 [2]南通大学江苏省神经再生重点实验室,南通226001

出　　处：《中国生物化学与分子生物学报》2005年第6期791-795,共5页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金项目资助(No.3027042)~~

摘　　要：为探讨tropic1808基因作用的分子机制,采用高密度寡核苷酸芯片(Affymetrix芯片)对表达tropic1808基因和空载体的PC12细胞株进行转录水平分析.基于获得的基因表达信息,对UCSC、TRANSFAC、NCBI等公共数据库进行检索,观察表达tropic1808基因导致PC12细胞株的基因表达谱变化.在检测的15866个目标基因中,855个基因表达上调,80个有显著比较意义,涉及包括粘附因子、离子通道、信号转导、细胞代谢等基因成员.其中包括多个细胞粘附因子及与细胞分化、神经发生和突触发生的有关基因.推测Tropic1808基因过表达可诱导PC12细胞株中粘附因子基因水平上调及与细胞分化、神经发生和突触发生相关的基因表达上调.To survey the molecular alterations in a broader way, Affymetrix genechip was used to investigate the possible changes of mRNA level in PC12 cells over expression tropic1808 gene by comparison to control cells. Of the 15866 examined genes and expressed sequence tags （ESTs）, the expression of 855 genes were up-regulated, and 80 of them significantly increased by two folds or more. These genes were involved in a number of distinct families, including cadherins, ion channels, signal transduction molecules, metabolism and others. Increased mRNA levels of CRABP Ⅱ, Cdh 1, Ascl 2, and Lgals 3 were further confirmed by RT-PCR. Our findings suggested that a number of cadherins were up-regulated and some genes concerning to cell differentiation, synaptogenesis and neurogenesis were also increased their expression in PC12 cells overexpressing tropic1808 gene.

关 键 词：tropic1808 基因芯片 表达谱 PC12细胞 

分 类 号：Q189[生物学—神经生物学]