天然浸出液对自分离白腐真菌产木质素降解酶的影响  

Effect of natural lixiviums on ligninolytic enzyme production of white rot fungus

作　　者：张晶[1] 李翠珍[1] 文湘华[1] 

机构地区：[1]清华大学环境科学与工程系环境模拟与污染控制国家重点实验室,北京100084

出　　处：《清华大学学报（自然科学版）》2005年第12期1629-1632,共4页Journal of Tsinghua University(Science and Technology)

基　　金：国家"八六三"高技术项目(2002AA649100);国家自然科学基金资助项目(50478010)

摘　　要：为提高木质素降解酶产量,使其应用于环境工程领域,在T ien&K irk培养基的基础上,添加玉米芯、柏树、桃树、玉兰和连翘等浸出液,在空气环境中,对白腐真菌F 2进行自由悬浮振荡培养。白腐真菌F 2能够大量产木质素过氧化物酶(L iP)和锰过氧化物酶(M nP)。添加天然浸出液后,L iP最高酶活为122.6 u/L,M nP最高酶活为135.4 u/L。在基础T ien&K irk培养基中培养则未检测出这两种酶的活性。在不同地区采集到的天然浸出液对两种酶的影响也不一样。结果表明,保持白腐真菌生长条件与天然生长条件相似,加入天然浸出液,可以促进产酶。Various methods were used to enhance manganese peroxidase and lignin peroxidase production in white rot fungus F2. Natural lixivium from maize core, cypress, peach, yulan or forsythia was added into the medium. The peak LiP activity was 122.6u.L^-1, and MnP peak activity was 135.4u.L^-1. No LiP or MnP activity was detected when the fungus grew in the basal Tien and Kirk liquor medium. Wood taken from various geographical areas apparently supported different levels of MnP and LiP production. The results indicate that culturing F2 in conditions similar to its natural habitate can promote ligninolyticenzyme production.

关 键 词：白腐真菌 天然浸出液 木质素过氧化物酶 锰过氧化物酶 

分 类 号：X172[环境科学与工程—环境科学]