人类口腔粘膜癌前损害发生发展过程中CDKN_2基因作用的研究　Ⅱ．P16蛋白表达与抑癌基因Rb蛋白表达的相互关系  

The Research of the Roles of CDKN_2 Gene in the Carcinogenesis of Human Oral Mucosa(Ⅱ). A Study on the Relationship Between P16 Expression and PRb Expression by Immunohistochemical Method

作　　者：程斌[1] 陈谦明[1] 罗刚[1] 李秉琦[1] 胡颖川[1] 周红梅[1] 段开文[1] 

机构地区：[1]华西医科大学口腔医学院口腔粘膜病学研究室,华西医科大学病理学教研室

出　　处：《华西口腔医学杂志》1996年第1期57-60,共4页West China Journal of Stomatology

基　　金：国家自然科学基金;国家教委博士点专项基金;美国纽约中华医学基金

摘　　要：视网膜母细胞用易感基因（Ｒｂ）是第一个被确认的抑癌基因，但Ｒｂ基因及其产物ＰＲｂ在口腔粘膜癌前损害癌变过程中的改变和表达未见阐述。本研究应用ＬＳＡＢ免疫组织化学染色技术观察到口腔粘膜癌前损害发生、发展过程中的各阶段组织中均存在ＰＲｂ在胞核和胞浆中的阳性反应，且胞核表达强于胞浆，并具有异质性；至口腔粘膜鳞状细胞癌阶段，ＰＲｂ的表达与鳞癌的分化程度有关；这些结果在一定程度上支持作者在Ｐ１６蛋白研究中的有关推论，Ｒｂ、ＰＲｂ在口腔粘膜癌前损害癌变过程中的改变及其作用值得进一步探讨。The retinoblastoma susceptibility gene(Rb) was the firstly identified tumor suppressor gene. But the expression of Rb and its product. PRb, in oral carcinogenesis has not been reported. It was found immunohistochemically that the positive reactions of PRboccurred in cellular nuclei and cytoplasms of tissues in each stage of oral carcinogenesis.The positive staining in nuclei was stronger than that in cytoplasms and positive patterns showed heterogenecity.It seemed that PRb expression associaseiated with the differential degrees of invasive squamous cell carcinomas. These results support to some extent our speculations from the research on P16 expression in oral carcinogenesis and indicate that it is necessary to study further the changes and effects of Rb gene and its product PRb on the development of oral carciongenesis.

关 键 词：PRB P16 口腔粘膜 癌前损害 免疫组化 CDKN2 

分 类 号：R739.850.2[医药卫生—肿瘤]