上皮性钙粘蛋白与肝细胞癌侵袭、转移的关系研究  

Relationship Between E-Cadherin and Invasion and Metastasis of Hepatocellular Carcinoma

作　　者：洪坚善[1] 彭浩[1] 段小娴[2] 

机构地区：[1]广西医科大学肿瘤医院胃肠乳腺外科 [2]广西医科大学肿瘤医院病理研究室

出　　处：《医学研究通讯》2005年第12期11-14,共4页Bulletin of Medical Research

基　　金：广西青年科技基金(0229029)

摘　　要：目的探讨上皮性钙粘蛋白(E-Cadherin)在人肝细胞癌(HCC)中的表达及其与肝癌侵袭、转移性的关系。方法选择56例有完整随访资料的原发性肝细胞癌及相应癌旁组织标本、20例正常肝组织标本,用 RT-PCR 方法检测 E-Cad-herin mRNA 的表达;用免疫组织化学方法检测 E-Cadherin 的表达。结果 1.E-Cadherin 在肝细胞癌组织中的表达低于癌旁组织和正常组织,差异有显著性意义(P<0.05);癌旁组织和正常组织中 E-Cadherin 的表达无显著性差异(P>0.05);2.E-Cadherin 在肝癌组织中的表达与术后复发时间呈正相关(P<0.05;R^2=0.786),与病理分期呈负相关(P<0.05;R^2=0.678);3.癌组织中 E-Cadherin 表达与肝外转移呈负相关(P<0.05;R^2=0.704);4.癌旁组织中 E-Cadherin 表达与术后复发时间呈正相关(P<0.05;R^2=0.742)。结论 E-Cadherin 与肿瘤的分化程度,侵袭转移能力,复发倾向相关,在临床中有望作为肿瘤分化、复发、转移的评估指标之一。Objective To investigate the association between E - cadherin expression of hepatocellular carcinoma （HCC） and its invasion and metastasis. Methods RT - PCR and immunohistochemieal analysis technology were used to detect E - cadherin mRNA expression and E - cadherin protein expression respectively in 56 paired HCC and non - HCC liver tissue samples and 20 normal liver tissue samples. Results 1. The level of E - cadherin expression in HCC was lower than that in adjacent non - HCC liver tissue and in normal liver tissue and the difference was statistically significant （P 〈 0.05）; the difference of E - cadherin expression between adjacent non - HCC liver tissue and normal liver tissue had no statistically significance （P 〉 0.05）; 2. E - cadherin expression in HCC had a positive correlation with recurrence after surgical treatment （P 〈 0.05, R^2 = 0.786） and a inverse correlation with pathological grade （P 〈 0.05, R^2 = 0.678）; 3. There was an inverse correlation between E - cadherin expression in HCC and extrahepatic metastasis （P 〈 0.05, R^2 =0.704）; 4. Significant positive correlations were found between E - cadherin expression in adjacent non - HCC liver tissue and recurrence after surgieal treatment （P 〈 0.05, R^2 = 0.742）. Conclusion E - cadherin had an association with degree of tumor differentiation, capability of invasion and metastasis and tendency of recurrence. It is expected that E - cadherin will be one of indexes of differentiation, recurrence and metastasis.

关 键 词：上皮性钙粘蛋白 肝细胞癌 侵袭 转移 癌旁组织 

分 类 号：R735.7[医药卫生—肿瘤] R73-37[医药卫生—临床医学]