射频治疗对荷H22肝癌小鼠脾淋巴细胞免疫功能的影响  

Impact of Radiofrequency on Splenocyte Immunity of Mice Bearing H22 Liver Cancer

作　　者：张俊平[1] 潘宏铭[1] 方勇[1] 黄丽朋 吴金民[1] 

机构地区：[1]浙江大学附属邵逸夫医院肿瘤内科 [2]洛阳轴承集团公司总医院外一科,河南洛阳471003

出　　处：《癌症》2006年第1期34-39,共6页Chinese Journal of Cancer

摘　　要：背景与目的:射频治疗(radiofrequencytherapy)不同于手术之处在于治疗后的肿瘤组织仍留于体内,这种差别对机体细胞免疫功能的影响有待深入研究。本研究目的是评价射频治疗荷H22肝癌小鼠后脾淋巴细胞增殖活性、细胞毒作用及Th1/Th2细胞因子漂移状况的变化。方法:24只BALB/c小鼠随机分为射频治疗组、手术治疗组、荷瘤对照组及正常对照组4组。MTT法检测小鼠脾淋巴细胞增殖活性,双色流式细胞技术检测脾淋巴细胞细胞毒作用,双抗体夹心ELISA法测定脾细胞培养上清中IL-2、IFN-γ、IL-4及IL-10细胞因子浓度,半定量RT-PCR测定脾细胞IL-2、IFN-γ、IL-4和IL-10mRNA相对含量。结果:射频治疗组小鼠脾淋巴细胞增殖活性和细胞毒作用显著高于正常对照组、手术治疗组及荷瘤对照组(P<0.05)。射频治疗组IL-2和IFN-γ浓度及mRNA表达水平显著高于其他3组(P<0.05),IL-4和IL-10浓度及mRNA表达水平显著低于其他3组(P<0.05)。手术治疗组IL-2、IFN-γ、IL-4和IL-10浓度及mRNA表达水平与正常对照组相比无显著性差异(P>0.05)。结论:射频治疗能够促进荷H22肝癌小鼠脾淋巴细胞活化、增殖,增强对同源肿瘤细胞的杀伤作用。射频治疗可以促进Th1类细胞因子IL-2和IFN-γ的表达。BACKGROUND ＆ OBJECTIVE： Radiofrequency （RF） can destroy local tumor tissue with less damage to the intervening tissue. The effect of RF on immune function of tumor-bearing body needs to be further investigated. This study was designed to evaluate the impact of RF on splenocyte immunity of mice bearing H22 liver cancer through detecting splenocyte proliferation, cytotoxicity, and expression pattern of cytokines T helper type 1 （Th1）/Th2. METHODS： A total of 24 BALB/c mice were randomized into 4 groups： RF, surgical resection, tumor-bearing control, and normal control groups. The proliferation of splenocytes was detected by MTT assay, and the cytotoxicity of splenocytes was assayed by double-color flow cytometry （FCM）. The concentrations of interferon-γ （IFN-γ）, interleukin-2 （IL-2）, IL-4, and IL-10 in splenocyte culture supernatants were detected by enzyme-linked immunoabsorbent assay （ELISA）, and mRNA levels of the cytokines in splenocytes were assayed by reverse transcription-polymerase chain reaction （RT-PCR）. RESULTS： The proliferation of splenocytes and mortality of H22 cells were significantly higher in RF group than in surgical resection group, normal control group, and tumor-bearing group （P〈0.05）. The concentration and mRNA level of IL-2 and IFN-γ were significantly higher in RF group than in surgical resection group, normal control group, and tumor-bearing group （P〈0.05）; the concentration and mRNA level of IL-4 and IL-10 were significantly lower in RF group than in surgical resection group, normal control group, and tumor-bearing group （P〈0.05）. No significant difference were found between surgical resection group and normal control group （P〉0.05）. CONCLUSIONS： RF can efficiently stimulate splenocyte activation and proliferation in H22-bearing mice, and enhance splenocyte cytotoxicity to tumor cells. RF could facilitate the secretion and gene transcription of Th1 type cytokines in H22 cells.

关 键 词：肝肿瘤 射频治疗 H22细胞 TH1细胞因子 TH2细胞因子 免疫功能 小鼠 

分 类 号：R735.7[医药卫生—肿瘤]