体外培养的兔睫状体色素上皮细胞表达转化生长因子-β_1  

Expression of TGF-β_1 in cultured rabbit ciliary pigment epithelial cells in vitro

作　　者：熊新春[1] 苗娟[1] 李庆林 席祖莲 张海江[1] 胡义珍[1] 魏厚仁[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院眼科,湖北省武汉市430022 [2]湖北省长阳市土家族自治县人民医院眼科,443500 [3]湖北省宜昌市夷陵医院眼科,443100

出　　处：《眼科新进展》2006年第1期12-13,共2页Recent Advances in Ophthalmology

基　　金：湖北省科技攻关项目(编号:2004AA301C37);国家教育部留学回国人员科研启动基金(编号:1999363)~~

摘　　要：目的研究体外培养条件下兔睫状体色素上皮(ciliarypigmentepithelium,CPE)细胞是否表达转化生长因子β1(transforminggrowthfactorβ1,TGFβ1),探讨TGFβ1在增殖性玻璃体视网膜病变中的作用。方法体外培养兔眼CPE细胞,取第3代细胞应用免疫组织化学Supervision染色方法检测TGFβ1的表达,用PBS代替第1抗体作为阴性对照。结果与正常活体组织不同,体外培养的兔CPE细胞经免疫组织化学染色,其细胞膜和细胞浆呈棕黄色阳性着色,阴性对照组未见明显棕黄色阳性着色。结论CPE细胞具有合成和表达TGFβ1潜能,为进一步研究增殖性玻璃体视网膜病变、房水形成和睫状体炎症奠定了基础。Objective To explore whether ciliary pigment epithelium （CPE） cells cultured fin vitro express transforming growth factor-β1 （TGF-β1） and to finvestigate the biological effect of TGF-β1 in proliferative vitroretinopathy. Methods Rabbit CPE cells were cultured in vitro and passaged three times, then the TGF-β1 expression was detected by immunohistochemistry methods. The negative control followed the same procedures except for the replacement of Ⅰ antibodies with PBS. Results TGF-β1 immunostaining were positive in the membrane and cytoplasm of cultured CPE cells, While no tmmunostaining was seen in the negative control. Conclusion CPE cells possess the potential ability to form and express TGF-β1. This phenomenon may be helpful to further study some eye diseases, such as proliferative vitroretinopathy, the secretion of aqueous humor and ciliary inflammation.

关 键 词：睫状体色素上皮 转化生长因子-Β1 兔 

分 类 号：R773.3[医药卫生—眼科]