气溶胶单粒子的检测效率与基质辅助激光解吸电离研究  

Investigation of Individual Aerosol Particles Detection Efficiency and Matrix Assisted Laser Desorption/Ionization

作　　者：周留柱[1] 顾学军[1] 朱元[1] 郭晓勇[1] 赵文武[1] 郑海洋[1] 方黎[1] 张为俊[1] 

机构地区：[1]中国科学院安徽光学精密机械研究所环境光谱学实验室

出　　处：《中国激光》2006年第1期97-101,共5页Chinese Journal of Lasers

基　　金：中国科学院创新方向性项目(KJCX2-SW-H08)资助课题

摘　　要：利用基质辅助激光解吸电离(MALDI)技术,在自行研制的气溶胶飞行时间质谱仪(ATOFMS)上实时探测单个生物气溶胶粒子的粒径和化学成分。采用双束激光空气动力学方法进行气溶胶粒子粒径的测量,并用266 nm的Nd∶YAG激光器对粒子进行激光解吸电离。以2,5-二羟基苯甲酸(DHB)和芥子酸为基质,对多肽生物样品进行了实验研究。质谱峰中质子化的分析物阳离子是在气相时,和基质通过质子转移反应形成的;而分析物碱金属加和物阳离子是在气相离子分子反应中,通过气相阳离子转移反应形成的。气溶胶粒子的检测效率为0.01%左右,命中率为10%左右。实验结果表明,该仪器可以满足大气中气溶胶单粒子的实时在线检测。The size and the chemical composition of an individual bioaerosol particle were detected in real-time by matrix-assisted laser desorption/ionization （MALDI） on the home built aerosol time of-flight mass spectrometer （ATOFMS）. The size of the aerosol particles was measured using two laser beams aerodynamic method, and the MALDI mass spectra were obtained using 266 nm pulsed Nd： YAG laser. Experiments were performed using polypeptide as analyte and 2,5-dihydroxybenzoic acid （DHB） and sinapic acid as matrices. The protonated analyte ion was generated by gas phase proton transfer with matrix, while the adducts ion of analyte was generated by gasphase cation transfer reaction with alkali metals ion. Detection efficiencies of aerosol particles were measured to be -0.01%, and the hit rates were measured to be -10%, showing that the ATOFMS is eligible for the real-time detection of aerosol particles in atmosphere.

关 键 词：光谱学 气溶胶飞行时间质谱仪 基质辅助激光解吸电离 检测效率 多肽 

分 类 号：O433.54[机械工程—光学工程]