小麦新品种“川麦42”低分子量谷蛋白亚基新基因的分子克隆  

Molecular Cloning of A Novel Low-molecular-weight Glutenin Subunit Gene from Wheat Variety "Chuanmai 42"

作　　者：沈蕾[1] 龙海[1] 颜泽洪[1] 魏育明[1] 郑有良[1] 

机构地区：[1]四川农业大学小麦研究所,都江堰611830

出　　处：《遗传》2006年第1期57-64,共8页Hereditas（Beijing)

基　　金：国家863计划(国家高技术研究发展计划项目)(编号:2003AA207100);高等学校全国优秀博士学位论文作者专项资金(编号:200357和200458);长江学者和创新团队发展计划(编号:IRT0453)~~

摘　　要：采用PCR方法从小麦(Triticum aestivumL.)新品种“川麦42”中克隆得到一个低分子量谷蛋白亚基(LMW-GS)新基因,暂命名为LMWCM42-1。该基因编码区全长846 bp,编码281个氨基酸,具有LMW-GS基因的典型结构特征。推导氨基酸序列比较显示,尽管LMWCM42-1与已知LMW-GS高度相似,但在N-末端重复区部分重复单元以及C-末端区中仍存在明显差异。聚类分析表明,LMWCM42-1可能是由Glu-D3位点编码的。Using PCR-amplification method, the coding sequences of a novel low-molecular-weight glutenin （LMW-GS） gene, LMWCM42-1, was isolated from the genomic DNA of wheat （Triticum aestivum L. ） variety ‘Chuanmai 42＇. The full coding region of this gene consisted of 846 nucleotides, and encoded a protein with 281 amino acids. LM- WCM42-1 is a typical low-molecular-weight glutenin subunit （LMW-GS） gene. In spite of high similarity with the known LMW-GSs, LMWCM42-1 was quite different from the known LMW-GS in the N-terminal, central repetitive domain and C-terminal domain. It was found that LMWCM42-1 was located within the Glu-D3 locus by phylogenetic analysis.

关 键 词：小麦 低分子量谷蛋白亚基基因 序列分析 

分 类 号：S512.1[农业科学—作物学]