抑胶素对C6胶质瘤细胞p16蛋白表达的影响  

Study the effect of Antigliomatin on protein of p16 in C6 glioma cells in vitro

作　　者：谢晓娜[1] 马涤辉[2] 林卫红[2] 崔俐[2] 王绍[3] 

机构地区：[1]吉林大学第一医院内分泌科,吉林长春130021 [2]吉林大学第一医院神经内科,吉林长春130021 [3]吉林大学白求恩医学部基础医学院生理教研室,吉林长春130021

出　　处：《中风与神经疾病杂志》2005年第6期515-516,共2页Journal of Apoplexy and Nervous Diseases

摘　　要：目的探讨不同浓度的抑胶素对C6胶质瘤细胞p16蛋白表达的影响。方法体外培养C6胶质瘤细胞,应用顺铂作为对照,通过免疫细胞化学染色法检测不同浓度的抑胶素作用后细胞周期负性调控基因p16在不同时问点的蛋白表达。结果 C6胶质瘤细胞经过抑胶素处理后,随着抑胶素浓度的增加,p16蛋白的表达也明显增强。结论抑胶素能够抑制C6胶质瘤细胞的增殖,并且具有改变肿瘤细胞增殖周期的作用。Objective To explore the influence of Antigliomatin on protein of p16 in C6 glioma cells. Methods C6 glioma cells were cultured in vitro. Immunohistochemical study was used to detect the expression of protein of p16 in C6 glioma cells treated by different concentration of Antigliomatin and cisplatin respectively. Results Antigliomatin can increase the expression of protein of p16 with dose-dependent tendency. Conclusion Antigliomatin can inhibit the growth of C6 glioma cells ,which may be related to the mechanism of its increasing the expression of protein of p16.

关 键 词：抑胶素 C6胶质瘤细胞 P16蛋白 

分 类 号：R739.41[医药卫生—肿瘤]