磁富集法检测52例胸腹腔积液中癌细胞的结果分析  

52 Detections of Cancer Cells from Peritoneal and Pleural Fluid Using Magnetic Enrichment Method

作　　者：楼兰英[1] 

机构地区：[1]浙江交通职业技术学院医院,311112

出　　处：《实用癌症杂志》2005年第5期495-497,共3页The Practical Journal of Cancer

基　　金：浙江省自然科学基金(M103028);浙江省教育厅重点项目(20041174)

摘　　要：目的探讨磁富集法对胸腹腔积液中癌细胞检测的临床价值。方法采用磁富集法检测52例患者体腔积液(胸腔积液20例,腹腔积液32例)中癌细胞,并与常规细胞检测方法进行比较。结果根据细胞学的检测结果进行分组,在细胞学诊断为恶性的11例患者中有10例用磁富集检测出恶性肿瘤细胞,4例细胞学诊断为不典型细胞的患者中用磁富集检测出2例恶性肿瘤细胞,38例细胞学诊断为阴性的患者中用磁富集检测出3例为恶性肿瘤细胞。共有5例在细胞学诊断为不典型细胞或阴性的患者中用磁富集检测出恶性肿瘤细胞,其中4例在别的检测方法得到证实,1例患者失访。52例病例中阳性率为38.5%,磁富集法的阳性检出率为85.0%,而常规方法的检出率仅为60.0%。结论磁富集法检测腹腔或胸腔积液中的恶性肿瘤敏感性高于常规细胞学检测,其结果有利于更准确的肿瘤分期。Objective To explore the clinic value of the magnetic enrichment method tests for detecting the cancer cells in peritoneal and pleural fluids. Methods The fluid specimens from 52 patients were examined to determine the frequency of recovery of epithelial cells compared with that achieved by conventional cytopathology（20 of the peritoneal fluid, 32 of the pleural fluid）. Resuits Grouping the cases by cytologic diagnosis,it is found that malignant cells using the cell enrichment assay in 10 of 11 malignant cases,2 of 4 atypical cases,and 3 of 38 negative cases. Further investigations were conducted on the 5 cases that were cytologically negative or atypical, but yielded epithelial cells after immunomagnetic enrichment. 4 cases ultimately were proven to be malignant by other methods and one had incomplete follow-up. The passive rate was 38.5% for the 52 patients. The checkout rate of the magnetic enrichment method was 85.0%. However, the checkout rate of the conventional cytopathology was 54.5 %. Conclusion The new methods available for epithelial cell enrichment in liquids may be used successfully on cytologic fluid specimens and may lead to increased sensitivity for detection of malignancy tumors, and consequently more accurate staging.

关 键 词：磁性粒子 细胞学诊断 磁富集诊断 癌细胞 

分 类 号：R730.53[医药卫生—肿瘤]