高粱A_2 CMS减数分裂染色体行为观察  

Chromosomal Meiotic Behaviors in A_2 Cytoplasmic-male Sterility in Sorghum

作　　者：梁小红[1] 仪治本[1] 赵威军[2] 崔贵梅 孙毅 崔丽霞[1] 

机构地区：[1]中北大学环境与安全工程系,山西太原030051 [2]山西省农业科学院高粱研究所,山西晋中030601 [3]山西省农业生物技术研究中心,山西太原030031

出　　处：《华北农学报》2005年第6期42-45,共4页Acta Agriculturae Boreali-Sinica

基　　金：山西省科技攻关项目(021011);中北大学自然科学基金项目(2005050)

摘　　要：高粱(Sorghum bicolorL.Moench)质核互作雄性不育有7种类型,即A1,A2,A3,A4,A5,A6和9E。以A2/B2V4为材料,对A2类型的雄性不育系进行了研究,结果表明:高粱A2V4 CMS花粉母细胞减数分裂染色体行为,在前期Ⅰ至中期Ⅰ无任何异常现象,从后期Ⅰ开始,染色体行为出现各种异常现象,如同源染色体在走向两极时出现滞后或部分不分离;或在后期Ⅱ姐妹染色体不分离造成细胞内染色体多倍化,以致形成两极染色体数目不等;在一个细胞内有几个染色体团或核;三分体及“T”型和直线型排列的四分体。所有这些异常现象最终导致减数分裂不能正常进行,不能发育正常的小孢子,因而造成雄性不育。Seven kinds of sorghum （Sorghum bicolor L. Moench） cytoplasmic-male sterility （CMS） have been identified by far, i.e. A1, A2, A3, A4, A5, A6 and 9E. The mechanic studies of CMS was mainly centered on Al（milo）. A2/BEV4 was used in our program to study the chromosomal meiotic behaviors during pollen mother cell development in A2 CMS. The results demonstrated that the abnormal chromosomal behaviors occurred in A2 CMS meiosis starting from anaphase Ⅰ ; Homologous chromosomes delayed to move to opposite poles at anaphase Ⅰ or sister chromosomes didn＇t divide at anaphase Ⅱ ; Chromosomes muhipolidized; Unequal chromosomal separation; Several nuclei or nucleoli in one cell; etc. All of the abnormal behaviors lead to the failed microspores development.

关 键 词：高粱 CMS 减数分裂 染色体行为 

分 类 号：S514.01[农业科学—作物学]