棉铃虫增殖SfaMNPV的产量试验  

PRODUCTION TRIAL OF SFAMNPV PROPAGATING IN LARVAE OF HELIOTHIS ARMIGERA

作　　者：朱顺义[1] 王家坤[1] 陈曲侯[1] 

机构地区：[1]华中师范大学昆虫学研究所,湖北农学院农学系

出　　处：《华中师范大学学报（自然科学版）》1996年第1期72-76,共5页Journal of Central China Normal University：Natural Sciences

摘　　要：用棉铃虫作为芹菜夜蛾核型多角体病毒的替代宿主，研究了影响该病毒产量的几个因素．结果发现，当３龄棉铃虫用４．９４×１０５ＰＩＢｓ／ｍＬ饲喂６０ｈ得到的ＰＩＢ产量最高．每头棉铃虫平均产量达到７．６５１×１０９ＰＩＢＳ．全程死亡天数１１ｄ．同时发现幼虫死亡体重与ＰＩＢ产量之间存在显著的直线回归关系，斜率为０．０１３７２，即死亡幼虫体重每增加１ｍｇ，ＰＩＢ数目就要增加１．３７２×１０７．在比较温度对ＰＩＢ产量影响的试验中，２６℃比３０℃更有利于病毒生产，其增殖曲线典型，有较长的对数增殖期，最终病毒滴度高．By studying the effect of virus dosage, inoculating time and larval instar on production of SfaMNPV propagating in its alternate host Heliothis armigera, we discovered that greatest production of virus occured by feeding 3rd-instar larvae on diet containing 4. 94 X 105 polyhedral inclusion bodies (PIBs )/mL ddH2Ofor 60 bars, and the average yield of PIB is 7.651 X109 PIBs per larvae. The mean number of days required for death to occur was 11 days. A highly significantrelationship was found between PIB production and larval-death weight, and the slope of the regression was 0.01272, showing that for each milligram increase in larval weight, there was an increase of 1.372 X 107 PIBs. By studying effect of temperature on the production and the growth dynamics of SfaMNPV in its alternate host larvae, we found 26C was fitter than 30℃ for virus to propagate,and showing typical propagation curve, longer logarithm period and high polyhedra titer.

关 键 词：芹菜 夜蛾 多角体病毒 棉铃虫 产量试验 增殖 

分 类 号：S476.13[农业科学—农业昆虫与害虫防治]