三氧化二砷逆转肺癌细胞株耐药的实验研究  

Studies on the reverse of resistance using arsenic trioxide incombination with DDP in HTB-56/DDP cell lines of lung cancer

作　　者：吴广洲[1] 郑世营[2] 朱天忠[1] 刘国锋[1] 于宏基[1] 

机构地区：[1]南通大学第四附属医院胸心外科,盐城224006 [2]苏州大学附属第一医院胸心外科

出　　处：《南通大学学报（医学版）》2005年第6期424-425,427,共3页Journal of Nantong University(Medical sciences)

摘　　要：目的:观察三氧化二砷(As2O3)与肺腺癌细胞株HTB-56、相应耐药细胞株HTB-56/DDP共孵育有无诱导细胞凋亡以及逆转耐药作用。方法:采用人肺腺癌细胞株HTB-56、HTB-56/DDP作为实验细胞,以As2O3和顺铂(DDP)作干预,用MTT法检测As2O3单独和与DDP共孵育后细胞的生长抑制率得半数抑制浓度得出抗药倍数;以电镜观察干预后细胞凋亡情况。结果:不同浓度As2O3作用相同时间或相同浓度作用不同时间对HTB-56、HTB-56/DDP的细胞抑制率有明显差异(P<0.05);HTB-56/DDP细胞株与HTB-56细胞株相比,对顺铂的抗性倍数为14.65倍;而与As2O3共孵育对DDP的抗药倍数则为6.35,逆转倍数约为3.5,As2O3对其有增敏及逆转耐药作用和诱导细胞凋亡。结论:As2O3有明显抗肿瘤作用,能增强肺癌耐药细胞株对DDP的敏感性,对逆转耐药有一定的作用。Objective ：To observe the effects of arsenic trioxide （ As2O3 ） and cisplatin （DDP） alone or in combination on reverse of resistance in HTB - 56/DDP cell lines and apoptosis - inducing effects of arsenic trioxide. Methods：The sensitivity of HTB -56/DDP cells to As2O3 and DDP was tested by means of MTT in vitro and the morphological changes of apoptosis were detected by electron microscopy. Results ：The resistance of HTB -56/DDP cell line to DDP was 14.65 times higher than that of HTB - 56, but the IC50 value of both cell lines to As2O3 was similar （ P 〈 0.05）. Cell inhibition by DDP combining with low - dose As2O3 was higher than that of administration alone. As2O3 could reinforce the sensitivity of HTB - 56/DDP cells to DDP and induce apoptosis of cell lines. Conclusion：As2O3 might be an effective anti -tumor drug as well as an effective drug for reversal of HTB -56/DDP to DDP.

关 键 词：肺癌 HTB-56 顺铂 三氧化二砷 耐药逆转 

分 类 号：R734.2[医药卫生—肿瘤]