盐渍肠衣猪瘟病毒实验室检测的相关性研究  

Study on Measuring Relationship in Laboratory for Detection of Classical Swine Fever Virus in Salted Swine Casings

作　　者：刘增再[1] 朱中武 唐连飞 胡述光[3] 刘毅[1] 徐霞[1] 李娜[1] 彭运潮[1] 

机构地区：[1]湖南农业大学动物科学技术学院,湖南长沙410128 [2]湖南省出入境检疫检验局技术中心,湖南长沙410007 [3]湖南生物机电职业技术学院,湖南长沙410127

出　　处：《湖南农业大学学报（自然科学版）》2005年第6期644-647,共4页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家质量检验检疫总局资助项目(2003D10509)

摘　　要：参考GenBank中发表的猪瘟病毒(CSFV)序列,设计一对CSFV特异性引物;从CSFV感染猪盐渍小肠中提取总RNA,经逆转录后进行PCR扩增,在盐渍小肠中成功扩增出与预期大小(168bp)一致的特异性条带,而正常猪和感染猪伪狂犬病病毒的猪小肠扩增结果均为阴性.用此方法对40例盐渍猪肠衣样本进行检测,结果与经典抗原检测方法(抗原捕获ELISA法)一致,而兔体交叉反应试验的阳性检出率为RT-PCR的66.7%,表明本RT-PCR技术能应用于盐渍猪肠衣的CSFV检测.A pair of specific primers was synthesized according to the sequences published in GenBank. The specific expected fragment （168 bp） was amplified by RT-PCR from the total RNA extracted from salted casings of pigs infected CSFV, whereas, no amplification were detected in normal or pseudorabies virus （PRV） infected. Forty salted swine casing samples were detected by this method, which showed the same results as antigen capture ELISA test did. Compared with RT-PCR, the positive rate of rabbit-cross reaction test was 66.7%. Therefore, this method could be applied to the fast and accurate detection of CSFV in salted swine casings.

关 键 词：逆转录聚合酶链式反应 酶联免疫吸附试验 兔体交叉反应试验 猪瘟病毒 盐渍肠衣 

分 类 号：S852.651[农业科学—基础兽医学]