大蒜油激活鼠肝枯否细胞抗癌作用的实验研究  

Garlic Oil Activate Anticarcinogenesis of Mouse Kupffer Cell in vitro

作　　者：张森[1] 万德森[2] 肖锡宾[2] 潘志忠[2] 高枫[1] 

机构地区：[1]广西医科大学第一附属医院大肠肛门病外科,南宁530021 [2]中山大学肿瘤医院腹科,广州510060

出　　处：《大肠肛门病外科杂志》2005年第4期257-259,共3页Journal of Coloproctological Surgery

摘　　要：目的:体外实验明确大蒜油是否能增强枯否细胞(KupfferCell,KC)的抗肿瘤活性。方法:酶消化法提取小鼠肝脏枯否细胞,分别以含大蒜油浓度为1200、600、300、150、75、37·5μg/mL的培养液培养枯否细胞24h。换洗培养液后,按10∶1效靶比加入小鼠结肠癌细胞CT26,共同培养24h。用MTT法测定活细胞数量。以未加药的KC/CT26共同培养和KC、CT26单独培养为对照组。结果:KC的杀癌率为10·49%。经一定浓度大蒜油激活后,KC的杀癌率明显增强,并且杀癌率随大蒜油浓度的升高而增强,大蒜油浓度分别为1200、600、300、150、75、37·5μg/mL的杀癌率分别为91·91%、75·92%、73·71%、42·38%、37·94%和18·07%。300μg/mL组与600μg/mL组差异不明显,37·5μg/mL组与不用大蒜油激活组亦无差异。结论:大蒜油能激活枯否细胞,从而增强其抗癌活性。300μg/mL的大蒜油浓度可能是一个恰当的选择。Objective：Kupffer Cell （KC） in liver is the maximum part of macrophage in vivo. This study is to determine the antineoplastic activity of KC strengthened by garlic extract in vitro. Methods：Kupffer cell of mouse abstracted by enzyme digestion were cultured with garlic oil contain 1200,600,300,150,75,37.5 μg/mL respectively. Mouse colon cancer cells （CT26） were joined together according to effect target ratiol of 10：1 after changing culture fluid. Viable cell were assayed with MTT and control groups were KC/ CT26 culture together and KC, CT26 culture alone. Results： The repress/ kill carcinoma rate of KC were 10.49% and could be enhanced by a certainty density of garlic oil distinctly. The repress/kill carcinoma rate came along with the garlic oil density as follows：91.91%,75.92% ,73.71%,42. 38% ,37. 94% and 18. 07 % with 1200,600,300,150,75 and 37.5μ g/mL respectively. No difference were found between 300μ g / mL group with 600μ g/mL group and 37. 5μg /mL group with no-medicine KC/CT26 culture together group. Conclusion：Garlic oil can activate Kupffer cell and enhance its anticancer activity. 300 μg/mL may be a correct select.

关 键 词：枯否细胞 大蒜 MTT 抗癌 

分 类 号：R73-36[医药卫生—肿瘤]