L-抗坏血酸处理辐照豌豆种子的细胞学效应  

Cytology Effect of Irradiated Pea with Different L-ascorbic Acid Concentration

作　　者：徐明照[1] 林青松[1] 钱艳[1] 黄训端[2] 田秋元[1] 

机构地区：[1]安徽农业大学生命科学学院,安徽合肥230036 [2]安徽大学生命科学学院,安徽合肥230036

出　　处：《安徽农业科学》2005年第12期2359-2360,2368,共3页Journal of Anhui Agricultural Sciences

摘　　要：豌豆种子经60Coγ射线辐照后,用L-抗坏血酸溶液浸泡,研究不同浓度L-抗坏血酸溶液处理辐照豌豆种子的细胞学效应。结果表明:经不同浓度的L-抗坏血酸处理,对辐照产生的染色体畸变率、微核细胞率、发芽率、成苗率、细胞分裂指数均有明显的影响。在较低浓度(<0.05mol/L)处理条件下,发芽率、成苗率、细胞分裂指数明显上升,染色体畸变率、微核细胞率呈下降趋势。在处理浓度为0.05mol/L时,效果明显;在较高浓度(>0.05 mol/L)处理条件下,其效应相应减弱。The seeds of pea were irradiated by different dose of ^60Coγ ray,which were soaked in the different eoneentrations of Vc solution and were studied in the sprout rate, miero-nuclear rate, mitosis index, chromosome aherration type and frequency. The results showed that：the root tip cells of pea could be aherrated by ^60Coγ ray. There was amazing function to the rate of sprout, seeding and cell mitosis by small concentration of Vc . With the irradiation dose increasing, inhihition was more obvious. Beta een 0-300 Gy, the aberration rate of chromosome and nuelear were redueed effeetively hy 0.05 mol/L concentration of Vc solution.

关 键 词：豌豆 ^60Coγ射线 L-抗坏血酸 细胞分裂指数 染色体畸变率 

分 类 号：S124.1[农业科学—农业基础科学]