血红蛋白分析对地中海贫血筛查的评估  

Assessment of the hemoglobin detection in the diagnosis of the thalassemia

作　　者：王剑飙[1] 吴洁敏[1] 石厚荣[1] 夏文权[1] 余文红[1] 倪培华[1] 胡翊群[1] 

机构地区：[1]上海第二医科大学瑞金临床医学院检验系,上海200025

出　　处：《诊断学理论与实践》2005年第6期462-464,共3页Journal of Diagnostics Concepts & Practice

摘　　要：目的:评估血红蛋白分析对地中海贫血(Thal)诊断能力。方法:用HLC-723G7自动血红蛋白分析仪对以临床表现流行病学资料和醋纤薄膜电泳等确定的115例Thal患者进行血红蛋白分析,结合分子诊断评估诊断价值。结果:以HbA2≥5.5%作为血红蛋白分析对β-Thal的诊断标准,则与DNA分析的符合率为81.5%;以HbA2≤2.5%为仪器对α-Thal的诊断标准,则与DNA分析的符合率为92.5%。结论:血红蛋白分析可提供α、βThal的诊断信息,若能结合更多的临床和DNA分析数据,可作为α、β地中海贫血的确诊手段。Objective To assess the hemoglobin analysis in the diagnosis of the thalassemia. Methods The hemoglobin detection was performed using HLC-723G7 analyzer in 115 patients diagnosed as thalassemia based on the clinical manifastations, the epidemiological findings and cellulose acetate membrane electrophoresis-elution. The diagnostic significance was assessed in combination with the DNA analysis. Results The patients with higher HbA2 value than 5.5% were conformed to 81.5% β-thalassemia diagnosed through the DNA analysis. The patients with lower HbA2 value than 2.5% were conformed to 92.5% α-thalassemia diagnosed through the DNA analysis. Conclusions The diagnosis of α or β- thalassemia may be confirmed through the automated hemoglobin analysis, the clinical manifestations and the DNA analysis.

关 键 词：血红蛋白分析 地中海贫血 血红蛋白A2 DNA分析 

分 类 号：R556.61[医药卫生—血液循环系统疾病] R446.11[医药卫生—内科学]