EphA2与E-cadherin在胰腺癌中的表达及其意义  

Expression and significance of EphA2 and E-cadherin in pancreatic carcinoma

作　　者：冯延平[1] 黄涛[1] 高军[1] 常青[1] 秦仁义[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院胆胰外科,湖北武汉430030

出　　处：《中国普通外科杂志》2005年第12期914-917,共4页China Journal of General Surgery

基　　金：国家自然科学基金资助项目(30271473)

摘　　要：目的探讨EphA 2和E-cadherin在胰腺癌组织及细胞中的表达及其临床意义。方法采用免疫组织化学SP方法,检测5 6例胰腺癌及2 3例癌旁非肿瘤胰腺组织中EphA 2和E-cadherin的表达,并分析其与临床病理因素的关系。采用RT-PCR方法检测EphA 2在胰腺癌细胞株BXPC-3,PC-3及PANC-1中的表达。结果胰腺癌组织中EphA 2阳性和E-cadherin阴性的表达率分别为6 6.0 7%和5 7.1 4%,均显著高于癌旁非肿瘤胰腺组织(P<0.0 5);EphA 2表达与胰腺癌分化程度、TNM分期和淋巴结转移有关,E-cadherin表达与胰腺癌TNM分期和淋巴结有关。在胰腺癌组织中,EphA 2与E-cadherin的表达呈负相关;EphA 2在胰腺癌高侵袭力细胞株BXPC-3和PANC-1呈高表达,在低侵袭力细胞PC-3呈低表达。结论EphA 2与E-cadherin的表达和/或功能异常可能共同参与胰腺癌的发生发展与转移;联合检测两种蛋白对于胰腺癌转移潜能的判断具有一定的参考价值。Objective To explore the expression and elinieal significance of EphA2 and E-cadherin in pancreatic cancer tissue and cells. Methods The expression of EphA2 and E-cadherin in 56 panereatie carcinomas and 23 adjacent noncancer tissues were studied bv immunohistochemistry, and their relationship to clinicopathological charaeteristies were analyzed. RT-PCR was performed to explore the expression of EphA2, BXPC-3, PC-3 and PANC-1 in pancreatic carcinoma cell lines. Results In pancreatic carcinomas tissuse showed inereased EphA2 expression and reduced E-cad expression which compared with adjacent non-cancer tissues. The expression level of EphA2 had a significant positive relationship with tumor differentiation degree, lymphatic metastasis and elinical stage. However, the expression level of E-eadherin had negative relationship with both the tumor clinieal stage and lymphatic invasion. Furthermore, a significant negative relationship between the expression of EphA2 and E-cadherin was obselwed. The expressions of EphA2 were higher in high-invasive celt lines PXPC-3 and Panc-1 than in low-invasive cell line PC-3. Conclusions The expression and/or abnormal function of EphA2 and E-eadherin may together be involved in the development and progression of pancreatie cancer; the combined measurement of these two proteins may be useful for determination of metastatic potency of pancreatic eareinoma.

关 键 词：胰腺肿瘤/病理学 EphA2 E-cadherin 癌基因蛋白质类 

分 类 号：R735.9[医药卫生—肿瘤] R730.231.3[医药卫生—临床医学]