正常雌鼠及6-羟基多巴型帕金森病雌鼠黑质听觉P50诱发电位的实验研究  

P50 auditory evoked potential in substantia nigra of normal female rats and female rat models of Parkinson disease induced by 6-hydroxydopamine

作　　者：孟金兰[1] 何敏[1] 罗海芸[1] 熊文昌[1] 魏书均[1] 

机构地区：[1]昆明医学院生理教研室

出　　处：《中国临床康复》2005年第48期68-70,共3页Chinese Journal of Clinical Rehabilitation

基　　金：云南省科委自然科学基金资助项目(2001C0005R)~~

摘　　要：目的:在清醒安静的自然状态下记录雌鼠黑质听觉P50诱发电位,同时观察6-羟基多巴胺对雌鼠黑质听觉P50诱发电位的影响。方法:实验于2003-01/2004-01在昆明医学院生理教研室神经电生理和帕金森病研究室进行。取成年雌性Sprague-Dawley鼠61只,在右侧黑质致密带埋置电极后随机分为3组:①模型组(n=25)雌鼠黑质致密带给予4μL的6-羟基多巴(2g/L),以0.5μL/min速度推注,注射完毕留针5min。②溶剂对照组(n=15)则向黑质致密带内注入同等剂量维生素C。③正常对照组(n=21)不给药。采用试验/条件(T/C)刺激模式,在清醒安静的自然状态下记录各组雌鼠黑质听觉P50诱发电位。结果:51只大鼠进入结果分析。①黑质内微量注射神经毒剂6-羟基多巴可成功建立帕金森病模型(皮下注射阿朴吗啡1mL/kg后,定向旋转>7r/min,持续时间>30min为造模成功);成功率60%(15/25)。②P50试验/条件(T/C)比值:正常对照组为66.68±3.71;溶剂对照组和正常对照组比较差异不显著(64.05±5.89,P>0.05),模型组显著低于溶剂对照组和正常对照组(39.90±5.41,P<0.01)。结论:①正常雌鼠黑质内存在听觉P50诱发电位。②多巴胺可能对黑质P50起着重要的调节作用。AIM： To record the P50 auditory evoked potential in substantia nigra of female rats in the quiet and awake natural states. Meanwhile, to observe the influence of 6-hydroxydopamine （6-OHDA） on the P50 auditory evoked potential in substantia nigra of female rats. METHODS： The experiment was carried out in the research rooms of neureelectrephysiology and Parkinson disease, the Department of Physiology of Kunming Medical College between January 2003 and January 2004. Sixty-one female Sprague-Dawley rats were embedded with recording electrode into right substantia nigra pars compacta, and then randomly divided into three groups： ① model group （n=25）： the substantia nigra pars eompaeta of the female rats were given 4 μL 6-OHDA （2 g/L） at a speed of 0.5 μL per minute, and the needle was kept for 5 minutes after injection. ② solvent control group （n=15）： Vitamin C of the same dosage was injected into substantia nigra pars compacta. ③ normal control group （n=21）： no administration was given. P50 auditory evoked potentials in substantia nigra were recorded in all the three groups by means of stimulation model of testing/conditioning （T/C）. RESULTS： Totally 51 rats were involved idn the analysis of results. ① 6-OHDA micro-injection in substantia nigra pars compacta can establish Parkinson disease model successfully （After subcutaneous injection of 1 mL/kg apomorphine, the oriented rotation 〉 7 round per mintes and lasted for 〉 30 minutes were taken as successful model establishment）, and the successful rate was 60% （25/50）. ② Testing/conditioning （T/C） ratio of P50 in the normal control group was 66.68 ±3.71, which had no significant diffenence from that in the solvant control group （64.05±5.89, P 〉 0.05）, and it was significantly lower in the model group than in the solvant control group and normal control group （39.90±5.41, P 〈 0.01）. CONCLUSION： ① P50 auditory evoked potential exists in the substantia nigra of normal female rats. ② Dop

关 键 词：帕金森病 诱发电位 听觉 黑质 羟基多巴胺类 

分 类 号：R742.5[医药卫生—神经病学与精神病学]