C_(60)-AEDP对H_(22)和S_(180)癌细胞生长及周期的影响  

Effect of C_(60)-AEDP to liver cancer cell of H_(22) and sarcoma cell of S_(180)

作　　者：张先稳[1] 刘永彪[2] 周强[3] 郝兴芝[3] 陈杰[3] 

机构地区：[1]苏北人民医院放疗中心,江苏扬州225001 [2]徐州医学院肿瘤研究所,江苏徐州221002 [3]徐州医学院医学影像系,江苏徐州221002

出　　处：《肿瘤防治杂志》2005年第20期1530-1534,共5页China Journal of Cancer Prevention and Treatment

基　　金：国家自然科学基金(30200069)

摘　　要：目的:探讨C60-AEDP对H22肝癌细胞和S180肉瘤细胞的影响及作用机制。方法:体外培养H22肝癌细胞和S180肉瘤细胞,MTT法观察药物在不同浓度、不同条件和不同时间下,对细胞生长方式的影响,FCM法了解其作用机制。结果:光照12h,C60-AEDP对H22和S180肿瘤细胞有生长抑制效应,呈浓度效应关系;在非光照条件下,C60-AEDP对H22和S180肿瘤细胞有生长抑制效应,呈浓度-时间效应关系;通过FCM观察,在光照条件下,C60-AEDP可引起G1/S周期阻滞;在非光照条件下,C60-AEDP可引起M/G1周期细胞阻滞;并都可引起细胞凋亡增加,H22、S180肿瘤细胞凋亡率在光照条件下分别增加了32%、26%,而非光照条件下分别增加了9%、5%。结论:在体外条件下,C60-AEDP在光照条件下和非光照条件下,均表现出对肿瘤细胞生长微弱的抑制效应。这种微弱的抑制效应可能是通过对细胞周期的影响而发挥作用。肿瘤防治杂志,2005,12(20)OBJECTIVE： To nvestigate the impact and mechanism of C60-AEDP to liver cancer cell H22 and sarcoma cell S180. METHODS： H22 and S180 cells were incubated at 96-hole board to observe effects of the drug on cell growth by MTT method, and to investigate the mechanism by FCM method. RESULTS： Under the illu mination condition for 12 h or not, it was demonstrated that C60-AEDP inhibited the growth of H22 and S180 cells, and the inhibitory rate was concentration- and time-dependents. C60 AEDP caused G1/S cycle block under illumination condition and M/G1 cycle block without illumination. The apoptosis rates of H22 and S180 cells were enhanced in the level of 32%, 260/00 under illumination condition, and 9%, 5% without illumination respectively. CONCLUSIONS： C60-AEDP has the slightly inhibitory effect on tumour cells of H22 and S180. It may be worked by the influence on cell cycle.

关 键 词：细胞周期 流式细胞术 肉瘤180 癌 肝细胞 

分 类 号：R73-3[医药卫生—肿瘤]