重组IFN-α蛋白联合endostatin基因对碱烧伤诱导角膜新生血管的抑制作用  

Inhibitory effect of recombinant IFN-α combined with endostatin gene on corneal neovascurization induced by alkali burn

作　　者：王晓燕[1] 袁中芳[2] 魏增涛[3] 马春红[1] 赵东[4] 张艳[1] 国纪林[2] 孙汶生[1] 

机构地区：[1]山东大学医学院免疫学研究所,山东济南250012 [2]济南市中心医院眼科,山东济南250013 [3]山东大学医学院,山东济南250012 [4]济南市中心医院病理科,山东济南250013

出　　处：《基础医学与临床》2005年第12期1119-1123,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(30100078);教育部高校博士点基金(20030422056)

摘　　要：目的探讨重组IFN-α蛋白联合endostatin基因对碱烧伤诱导角膜新生血管的抑制作用。方法兔眼球结膜下注射包有绿色荧光蛋白表达载体的脂质体,3 d后用激光共聚焦显微镜观察角膜绿色荧光蛋白表达。利用碱烧伤法制备兔眼角膜新生血管模型,球结膜下联合注射重组IFN-α蛋白及包有endostatin真核表达载体的脂质体,用裂隙灯显微镜观察对新生血管的抑制作用。结果实验组角膜有很强的绿色荧光,而在对照组则无荧光。联合应用重组IFN-α蛋白和endostatin基因治疗,第7、10、13天角膜新生血管长度、面积明显小于重组IFN-α蛋白和endostatin基因的单独治疗组(P<0.05)。结论重组IFN-α蛋白联合endostatin基因可有效抑制碱烧伤诱导角膜新生血管的生长。Objective To investigate the inhibitory effect of recombinant IFN-α combined with endostatin gene on corneal neovascurization induced by alkali bum. Methods Lipofectin with GFP expression vector was injected into subconjunctival tissue in eye of rabbit. Three days later, the expression of GFP in cornea was observed with a laser confocal microscope. CNV animal model was developed by alkali bum, recombinant IFN-α and lipofectin with endostatin expression vector were injected into the subconjunctival tissue in eye of rabbit. The inhibitory effect of them on corneal neovascurization induced by alkali bum was observed with slitlamp microscopy. Results There was expression of GFP in the cornea of experimental group, but not in the cornea of control group. Combining recombinant IFN-α with endostatin gene to treat corneal neovascurization, the results showed the lengths and areas of corneal neovascurization on the 7th, 10thand 13rd day in combined treatment group were significantly less than that in recombinant IFN-α treatment group and endostatin gene treatment group （ P 〈 0.05）. Conclusion Recombinant IFN-α combined with endostatin gene can effectively inhibit corneal neovascurization induced by alkali bum.

关 键 词：IFN-Α ENDOSTATIN 基因治疗 角膜新生血管 碱烧伤 抑制作用 

分 类 号：R772.2[医药卫生—眼科]