E-cadherin的表达与肝细胞癌侵袭和转移的关系  

Relationship between expression of E-cadherin and invasion and metastasis of hepatocellular carcinoma

作　　者：洪坚善[1] 彭浩[1] 段小娴[2] 

机构地区：[1]广西医科大学肿瘤医院胃肠乳腺外科,广西南宁530001 [2]广西医科大学肿瘤医院病理研究室,广西南宁530001

出　　处：《基础医学与临床》2005年第12期1139-1142,共4页Basic and Clinical Medicine

基　　金：广西青年科技基金(0229029)

摘　　要：目的研究上皮性钙黏蛋白(E-cadherin)在人肝细胞癌(HCC)中的表达情况,探讨其与肝癌的相关性。方法选择56例有完整随访资料的肝细胞癌及相应癌旁组织标本、20例正常肝组织标本,用RT-PCR方法检测E-cadherinmRNA的表达;用免疫组织化学方法检测E-cadherin的表达。结果①E-cadherin在肝细胞癌组织中的表达显著低于癌旁组织和正常组织(P<0.05),而在癌旁组织和正常组织中的表达无差异;②E-cadherin在肝癌组织中的表达与术后复发时间呈正相关(P<0.05),与病理分期呈负相关(P<0.05);③癌组织中E-cadherin表达与肝外转移呈负相关(P<0.05);④癌旁组织中E-cadherin表达与术后复发时间呈正相关(P<0.05)。结论E-cadherin表达缺失或下调与肝癌的分化程度、侵袭转移能力和复发倾向相关,对肝癌临床转归的评估有一定指导意义。Objective To investigate expression of E-cadherin in hepatocellular carcinoma （HCC） and discuss their pertinence. Methods RT-PCR and immunohistochemical analysis technology were used to detect E-cadherin mRNA expression and E-cadherin protein expression respectively in 56 paired HCC and non-HCC liver tissue samples and 20 normal liver tissue samples. Results 1. The level of E-cadherin expression in HCC was significantly lower than that in adjacent non- HCC liver tissue and in normal liver tissue （ P 〈 0.05 ） ; but no difference was seen between adjacent non- HCC liver tissue and normal liver tissue; 2. E-cadherin expression in HCC had a positive correlation with recurrence after surgical treatment（ P 〈 0.05） and an inverse correlation with pathological grade（ P 〈 0.05）; 3. There was an inverse correlation between E-cadherin expression in HCC and extrahepatic metastasis（ P 〈 0.05）; 4. Significant positive correlations were found among E-cadherin expression in adjacent non-HCC liver tissue and extrabepatic metastasis （P 〈 0.05 ）. Conclusion Loss and downregulation of E-cadherin have an correlation with degree of tumor differentiation, capability of invasion and metastasis and tendency of recurrence, which may have some instructive significance for the assessment of outcome of HCC.

关 键 词：上皮性钙黏蛋白 肝细胞癌 侵袭 转移 癌旁组织 

分 类 号：R735.7[医药卫生—肿瘤]