人参皂甙Rg3抑制小鼠肝癌淋巴道转移作用及其对免疫功能的影响  

Inhibition of the lymphatic metastasis and influence on immune functions in mice treated by ginsenoside Rg3

作　　者：赵翌[1] 刘基巍[1] 陈雅敏[1] 朱青山[1] 吕申[2] 

机构地区：[1]辽宁大连大连医科大学附属第一医院肿瘤科,116011 [2]大连医科大学基础医学院,116023

出　　处：《临床肿瘤学杂志》2005年第6期610-612,615,共4页Chinese Clinical Oncology

摘　　要：目的:观察人参皂甙Rg3抑制小鼠肿瘤生长及抗淋巴结转移的作用及对免疫功能的影响。方法:以Hca-F25//6A3-F(F)接种于615近交系小鼠,建立肝癌淋巴道转移动物模型,分为5组:Rg3预防组(接种肿瘤前给Rg3)、Rg3治疗组(Rg3)、阳性对照组(PDD)、联合治疗组(Rg3+PDD)和阴性对照组(生理盐水),比较各组的原发瘤抑制率和转移淋巴结抑瘤率,并通过流式细胞仪方法分析各组免疫指标CD4/CD8的变化。结果:联合治疗组原发瘤的抑瘤率明显提高,与阴性对照组相比具有统计学上明显差异(P<0·05);淋巴结转移抑制率含Rg3组均较阴性对照组高;Rg3预防组和Rg3治疗组CD4/CD8比值与阴性对照组相比升高,具有统计学上明显差异(P<0·05)。结论:人参皂甙Rg3具有明显的抗肿瘤作用,可以增强化疗药物PDD的抗癌作用,及抑制淋巴道转移作用,并提高荷瘤小鼠免疫功能。Objective：To observe the inhibition of lymphatic metastasis of tumor and influence on immune functions in tumor mice induced by ginsenoside Rg3 treatment. Methods：The transplanted tumor lymphatic metastasis model was established with Hca-F25//6A3-F（F） in 615 inbred mice, and the tumor mice were divided into five groups：treated with ginsenoside Rg3 ahead,ginsenoside Rg3 ,povitive group（cisplatin） , ginsenoside Rg3＋cisplatin, and control group（saline）, observing inhibition ratio of the primary tumor and metastatic lymph node,and CD4/CDs ratio. Results：The inhibition ratio of the primary tumor was significantly higher in the ginsenoside Rg3＋ cisplatin group ; the inhibition ratio of metastatic lymph node was higher in ginsenoside Rg3-containing group, comparing with the control group. And CD4/CDs was increasing in the ginsenoside Rg3 containing group. Condusion：Ginsenoside Rg3 play a role in anti-lymphatic metastasis,enhancing anti-tumor effect of cytotoxic drug,modulating the immune system.

关 键 词：人参皂甙RG3 Hca-F25//6A3-F(F) 淋巴道转移 动物模型 免疫功能 

分 类 号：R730.53[医药卫生—肿瘤]