非小细胞肺癌中DPC4基因的表达与微血管形成的关系  

Expression of DPC 4 gene in non-small cell lung carcinoma and its relation to angiogenesis

作　　者：吴绍新[1] 官德元[1] 张敬芳[1] 何方[1] 柯尊福[2] 夏东[2] 陈德基[2] 

机构地区：[1]湖北荆门荆门职业技术学院医学院病理学教研室,448000 [2]武汉大学医学院病理学教研室,430071

出　　处：《临床肿瘤学杂志》2005年第6期633-636,共4页Chinese Clinical Oncology

摘　　要：目的:探讨DPC4(deletedinpancreaticcarcinomalocus4,DPC4)基因在非小细胞肺癌(NSCLC)中的表达及其与微血管形成的关系。方法:利用免疫组织化学S-P法检测52例NSCLC组织、19例相应的癌旁正常肺组织中DPC4、VEGF的表达,用CD34标记血管内皮细胞并计算微血管密度MVD值。结果:DPC4在肺癌原发灶中的阳性表达率为63·5%(33/52),与癌旁正常肺组织中的阳性表达率89·5%(17/19)相比,DPC4阳性表达水平显著降低(P<0·05);DPC4与组织学类型、肿瘤细胞分化程度无关(P>0·05),但与淋巴结转移显著相关(P<0·05)。52例NSCLC中,DPC4的表达与VEGF、MVD值均呈负相关(r=-0·303,P=0·020)。结论:DPC4的低表达可能是肺癌发生过程的早期事件,可促进肺癌的淋巴结转移,并可通过直接或间接的作用促进肺癌血管生成。Objective ：To study the expression of DPC 4 （ deleted in pancreatic carcinoma locus 4, DPC 4 ） in non-small cell lung carcinoma as well as its association with angiogenesis. Methods ：The expression of DPC 4 ,VEGF and CD34 was detected in 52 cases with primary NSCLC and 19 adjacent normal lung tissues by using immunohistochemical S-P method. Results： Positive expression rate of DPC 4 in primary lung cancer tissues was 63.5% （33/52） ,compared with the positive rate 89.5% （ 17/19）in adjacent normal lung tissues, the DPC 4 expression level was significantly degraded （ P 〈 0.05 ）. The positive expression of DPC 4 had no correlation with tissue types and ceLLuLar differentiation （ P 〉0.05 ） ,but it was cLoseLy associated with Lymph node metastasis （ P 〈0.05 ）. In aLL 52 patients with NSCLC, the relationships between DPC 4 and VEGF or MVD, showed apparently negative correlation （ r=-0. 303, P=0.020）. Conclusion：The low expression of DPC 4 could be an early event during the course of the NSCLC＇s development. Simultaneously,it also promoted lymph node metastasis by promoting the angiogenesis in NSCLC derectly or indirectly.

关 键 词：非小细胞肺癌 DPC4 免疫组化 血管生成 

分 类 号：R734.2[医药卫生—肿瘤]