辅酶Q10对多巴胺损伤的PC12细胞凋亡的影响  

Influence of coenzyme Q10 on PC12 apoptosis injured by Dopamine

作　　者：李熙东[1] 赵春玉[1] 隋汝波[1] 高永良[2] 赵宝东[3] 刘兴 

机构地区：[1]锦州医学院附属第一医院神经内科,辽宁省锦州市121000 [2]锦州市中心医院,辽宁省锦州市121000 [3]锦州医学院解剖学教研室,辽宁省锦州市121000 [4]锦州市红十字血站,辽宁省锦州市121000

出　　处：《中国临床康复》2005年第45期32-33,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:观察辅酶Q10在以PC12细胞建立的多巴胺神经元凋亡细胞模型中的作用。方法:实验于2003-04/2004-04在锦州医学院科技试验中心进行。取体外培养的PC12细胞分为3组:①辅酶Q10组:加450μmol/L辅酶Q10和0.3mmol/L多巴胺。②多巴胺组:加0.3mmol/L多巴胺。③空白对照组:只加等量RPMI1640。24h后进行流式细胞仪测试DNA含量,检测细胞凋亡率。结果:多巴胺组细胞凋亡率明显高于空白对照组[(30.66±1.90)%,(0.82±0.07)%,P<0.01],辅酶Q10组细胞凋亡率[(16.05±2.16)%]明显低于多巴胺组(P<0.01)。结论:辅酶Q10能显著降低多巴胺引起的PC12细胞凋亡,提示辅酶Q10作为神经保护性药物对保护多巴胺能神经元,防治帕金森病可能有实际应用价值。AIM：To observe the effect of coenzyme Q10 （Co Q10） on the model of dopaminergic neuron apoptosis based on PC12 cell. METHODS：The experiment was done in the Experimental Center of Science and Technology at Jinzhou Medical College from April 2003 to April 2004.The PC12 cells, cultured in vitro, were divided into 3 groups：（1） Co Q10 group：450 μmol/L Co Q10 and 0.3 mmol/L Dopamine （DA） was added to culture medium; （2）DA group：0.3 mmol/L DA was added to culture medium; （3）Control group：RPM11640 of same dose was added to culture medium. After 24 hours, the content of DNA was tested with flow cytometer and the apoptosis rate was detected. RESULTS：The apoptosis rate in groups of Co QlO [（16.05±2.16）%] and Control [（0.82±0.07）%] were remarkably lower than that of DA group [（30.66±1.90）%]（P 〈 0.01 ）. CONCLUSION：Co Q10 can significantly reduce the PC12 apoptosis induced by DA. It is indicated that Co Q10, as a neuroprotective drug, possibly has an application value in the protection of dopaminergic neuron and the treatment of Parkinson disease.

关 键 词：帕金森病 泛醌 PC12细胞 细胞凋亡 

分 类 号：R742.5[医药卫生—神经病学与精神病学]