天泰1号对自发阿尔茨海默病鼠海马CA1区分子层突触可塑性的影响 超微结构定量研究(英文)  

Influence of tiantai No. 1 recipe on synaptic plasticity of the hippocampal CA1 region of spontaneous Alzheimer disease models Quantitative research of ultrastructure

作　　者：李明 吴正治 李耀芳 张永锋 贾秀琴 陈嫚茵 

机构地区：[1]深圳市中西医结合研究所,广东省深圳市518031

出　　处：《中国临床康复》2005年第45期182-184,共3页Chinese Journal of Clinical Rehabilitation

基　　金：广东省优秀中青年专家科研目标责任项目(98036)~~

摘　　要：背景:中枢神经的突触可塑性是行为依赖性学习记忆的核心基础,治疗阿尔茨海默病的天然中药是否通过增加突触可塑性来改善学习记忆尚罕见报道。目的押观察天泰1号对自发阿尔茨海默病模型学习记忆功能及神经突触可塑性的影响。设计:随机对照实验。单位:深圳市中西医结合研究所。材料:实验在深圳市中西医结合临床研究所二级动物实验室完成。实验动物为昆明种小鼠。方法押从21个月龄昆明种小鼠筛选出自发阿尔茨海默病(记忆障碍)鼠52只,随机分为4组即老年痴呆组、西药对照组、天泰1号6.80g/kg组、天泰1号20.41g/kg组,另设13只老年学习记忆正常鼠为老年正常组。西药对照组给以甲磺酸二氢麦角碱0.6mg/kg,天泰1号6.80g/kg,20.41g/kg组分别予天泰1号方6.80g/kg及20.41g/kg,以上均研配成0.5mL液体灌胃给药,连续60d,老年正常组和老年痴呆组均灌以等量双蒸水。用跳台实验检测学习记忆成绩鸦海马CA1区脑组织超薄切片,透射电镜观察,并全自动显微图像分析系统定量检测突触可塑性参数。主要观察指标:①天泰1号不同剂量对自发阿尔茨海默病鼠学习记忆的影响。②突触电镜观察及体视学定量检测结果。结果押实验过程中实验动物无脱失,均进入结果分析。①天泰1号不同剂量对自发阿尔茨海默病鼠学习记忆的影响:天泰1号6.80g/kg组,20.41g/kg组的学习、记忆错误次数均小于老年痴呆组,且天泰1号20.41g/kg组的学习、记忆错误次数小于天泰1号6.80g/kg组;天泰1号6.80g/kg组,20.41g/kg组的学习训练逃避潜伏期小于老年痴呆组,记忆测试安全平台潜伏期大于老年痴呆组,且天泰1号20.41g/kg组的记忆测试安全平台潜伏期大于天泰1号6.80g/kg组。②突触电镜观察及体视学定量检测:与老年正常鼠相比,老年痴呆鼠出现突触数明显减少,部分突触间隙模糊不清,突触连接间断,突触小泡大小不等BACKGROUND： The synaptic plasticity of central nerves is the key basis of behavior-dependent learning and memory, there have been few reports about weather the Chinese herbal medicine for treatment of Alzheimer disease is through enhancing synaptic plasticity to improve learning and memory. OBJECTIVE： To investigate the effect of tiantai No. 1 recipe on learning and memory function and synaptic plasticity of spontaneous Alzheimer disease models. DESIGN： A randomized and controlled trial. SETTING： Institute of Combination of Traditional Chinese Medicine and Western Medicine of Shenzhen. MATERIALS： The experiment was completed at the grade Ⅱ Laboratory of Institute of Combination of Traditional Chinese Medicine and Western Medicine of Shenzhen City. The experimemal animals were mice of Kunming strain. METHODS： Totally 52 Kunming mice, aged 21 months, with spontaneous Alzheimer disease （memory impairment） were chosen, and at random divided into 4 groups： senile dementia, western drug control, tiantai No. 1 recipe 6.80 g,/kg and 20.41 g/kg groups; besides 13 mice with normal learning and memory power were chosen as normal senile group. Hydergine 0.6 mg/kg was given for mice in western drug control group, tiantai No. 1 recipe 6.80 g/kg and 20.41 g/kg were respectively given for mice in tiantai No. 1 recipe 6.80 g/kg and 20.41 g/kg groups. The medicines above were all prepared into 0.5 mL solution and given by garage, for consecutive 60 days. The mice in normal senile and senile dementia groups were given double distilled water of the same volume. The learning memory power was tested by jumping stand; ultrathin cerebral section of hippocampal CAI region was examined with transmission electron microscope, and some parameters of synaptic plasticity was also quantitatively detected with automatic micrographic analysis system. MAIN OUTCOME MEASURES： （1） The effect of tiantai No. 1 recipe in differem doses on learning and memory of spontaneous Alzheimer disease mice. （2） Observation

关 键 词：阿尔茨海默病 神经元可塑性 长时程增强 大脑皮质 海马 学习 记忆 小鼠 

分 类 号：R749.1[医药卫生—神经病学与精神病学]