香石竹ACC氧化酶基因克隆及其反义表达载体构建  

CLONING OF ACC OXIDASE GENE OF CARNATION AND CONSTRUCTION OF ITS PLANT ANTISENSE EXPRESSION VECTORS

作　　者：刘会超[1] 李永春[2] 巨关生[3] 孙振元[3] 柴德勇 

机构地区：[1]河南科技学院,河南新乡453003 [2]河南农业大学,河南郑州450002 [3]中国林业科学研究院林业研究所,北京100091 [4]河南北方花卉集团公司,河南鄢陵461200

出　　处：《核农学报》2005年第6期461-464,473,共5页Journal of Nuclear Agricultural Sciences

基　　金：国家科技部转基因专项:香石竹抗衰老转基因株系的创建及其产业化(JY04-B-02)

摘　　要：据已报道的香石竹氨基环丙烷羧酸(ACC)氧化酶基因的cDNA序列设计特异引物,以香石竹品种“Master”基因组DNA为模板,用PCR方法克隆出香石竹ACO基因的部分序列。测序结果显示,克隆的序列与已报道序列基本一致。将克隆的香石竹ACO片段反向连接到植物表达载体pCAMBIA 1301中CaMV 35S启动子的下游,构建了香石竹ACO基因的反义表达载体pCAM-ACO2,为进一步通过反义技术延长转基因香石竹的花期奠定了基础。A pair of specific primers was designed according to the reported cDNA sequence of the DC-ACO （ Dianthus caryophyllus L. ACC Oxidase）, and a partial sequence of the gene was amplified from genomic DNA of a carnation variety ＇Master＇ by PCR. Sequence analysis showed that the cloned and the reported sequences were basically coincident. An antisense expression vector, named pCAM-ACO2, of the DC-ACO gene was constructed, in which the antisense sequence was controlled by the CaMV 35S promoter. The work laid the foundations of the future transgenic research for prolonging the flowering period of the transgenic carnation via antisense technology.

关 键 词：香石竹 ACC氧化酶基因 反义表达栽体 

分 类 号：S682.19[农业科学—观赏园艺]