HSPgp96体外诱导腹腔巨噬细胞抗肿瘤细胞毒作用  

The Cytotoxic Effect of Peritoneal Elicited Macrophages Induced by HSPgp96 on Anti-Tumor in vitro

作　　者：施海燕[1] 顾君一[2] 张天一[2] 林琳[2] 朱昌来[2] 

机构地区：[1]南通大学基础医学院病理生理学教研室,南通226001 [2]南通大学神经再生重点实验室,南通226001

出　　处：《中国肿瘤生物治疗杂志》2005年第4期272-274,共3页Chinese Journal of Cancer Biotherapy

基　　金：江苏省教育厅基金(03KJD320183)

摘　　要：目的：探讨HSPgp96对小鼠PEMφ体外抗肿瘤细胞毒作用的影响。方法：分离巯基乙酸盐诱导的小鼠PEMφ,随机分为培养液对照组、LPS诱导组、HSPgp96诱导组。应用硝酸还原酶法、MTT法、扫描电镜分别观测PEMφNO的生成、对肝癌细胞H22的细胞毒作用及形态学变化。结果：在HSPgp96的体外诱导下,小鼠PEMφNO的生成量显著增加,对肝癌细胞H22的细胞毒作用明显增强,其效应与LPS相当。结论：HSPgp96可在体外有效激活PEM中的抗肿瘤细胞毒作用,NO是PEMφ杀瘤机制中的重要效应分子之一。Objective： To evaluate the cytotoxic effect of PEMФ induced by HSPgp96 on anti-tumor in vitro. Methods： PEMФ separated from mice induced by thioglycolate were divided into three groups randomly： Culture medium in control; LPS-induced group; HSPgp96-induced group. The production of NO, the cytotoxic effect to H22 cells and the morphologic change of PEMФ were investigated separately by enzyme method, MTT assay and scanning electron microscope. Results： In vitro, HSPgp96 can increased NO production from PEMФ of mice and significantly enhance the cytotoxic effect of PEMФ to H22 cells as well as LPS. Conclusion： HSPgp96 can effectively induce the cytotoxic effect of PEMФ on anti-tumor in which NO is one of the capital effective molecules in vitro.

关 键 词：热休克蛋白GP96 腹腔巨噬细胞 肿瘤 细胞毒作用 

分 类 号：R730.51[医药卫生—肿瘤]