4500份脐带血造血干细胞保存研究  

Studies of 4500 Umbilical Cord Blood Units Banking

作　　者：韩俊领[1] 李茜[1] 杨丛林[1] 陆敏[1] 韩忠朝[1] 邱录贵[1] 

机构地区：[1]天津市脐带血造血干细胞库

出　　处：《中国肿瘤生物治疗杂志》2005年第4期285-288,共4页Chinese Journal of Cancer Biotherapy

基　　金：美国纽约中华医学基金(CMB)(1-748);天津市社会发展计划重大项目(003119811)

摘　　要：目的：制备和保存满足临床造血干细胞移植要求的脐带血造血干细胞。方法：按标准操作规程(SOP)进行脐带血的采集、分离、冷冻保存和检测。结果：在采集的6232份脐带血中,采集的净血量、TNC、CD34^+细胞百分率和CFU-C产率均值分别为90．7±22．2ml,10．5±3．4×10~8,0．24±0．15％,78．9±44．14／10~5NC．已保存符合标准的脐带血4500份；分离后TNC回收率超过80％,而CD34^+细胞、CFU-C回收率则超过90％,细胞活率无明显改变；冻存融化后细胞回收率80％～110％,细胞活率>90％．在接受569例无血缘关系患者查询中,93％患者可查找到≥4个HLA-A,B和DRB1位点相合的脐带血。结论：可为临床造血干细胞移植提供合格的脐带血造血干细胞。Objective： To prepare and cryopreserve umbilical cord blood （UCB） units for clinical hematopoietic stem cell transplantation. Methods： HSC of UCB units that met our standards were collected, separated cryopreserved and tested. Results： 4 500 （72.2%） of 6 232 cord blood units were suitable for preservation. Average net volume, numbers of total nucleated cells, percentage of CD34^＋ cells and CFU-C of the UCB units were 90.7 ± 22.2 ml, 10.5 ± 3.4 × 10^8, 0. 24 ±0. 15% , 78.9 ±44.14/10^5 NC, respectively. After separation, the cell recovery of nucleated cells, CD34^＋ cells and CFU-C were 82.1 ±6.52%, 92.5 ±6.15% and 90 ±9.67%, respectively. The cell viability after separating was over 95%. After thawing, the recovery of nucleated cells, CD34^＋ cells and CFU-C were 84.2 ± 10.1%, 96.5 ±21.8% and 115.1 ±23.3%, respectively. The cell viability was 93.8 ±4.4%. Among the 569 searching cases , 532 （93%） cases could found 4/6 of HLA-A, B and DR loci matched UCB units in our bank, including 39 （6.58%） found 6 loci matched and 237 （41.65%） found 5 loci matched. Conclusion： These results indicated that we can supply high quality cord blood HSC for clinical transplantation.

关 键 词：造血干细胞 脐带血 造血干细胞移植 标准操作规程 

分 类 号：R73-33[医药卫生—肿瘤] R318.12[医药卫生—临床医学]