粉唑醇GC和HPLC分析方法  

Study on the quantitative analysis method for flutriafol by GC and HPLC

作　　者：魏光[1] 慕卫[1] 杨连来[1] 潘金菊[1] 刘彦良[1] 

机构地区：[1]山东农业大学植物保护学院,山东泰安271018

出　　处：《精细化工中间体》2005年第6期39-40,共2页Fine Chemical Intermediates

摘　　要：采用气相色谱法和液相色谱法分别对粉唑醇进行了定性和定量分析。气相色谱法中以正二十烷为内标物,色谱柱采用3%OV-101 chromosorb AW DMCS 150-177μm1m×3mm玻璃柱,柱温为195℃,汽化温和检测温均为230℃,方法回收率为99.2%～100.9%,变异系数为0.262%。高效液相色谱法中Nova-PaK C18 250 mm×4.6mm色谱柱,以v(甲醇)+v(水)=851∶5为流动相,检测波长为262nm。方法回收率为98.9%～99.4%,变异系数为0.505%。The quantitative analysis method for flutriafol by GC and HPLC was studied. In the gas chromatographic method a 3%OV-101 on Chromosorb AW DMCS 150-177μm 1m×3mm （i.d.）, glass column was used at column temperature of 195℃ and detector temperature of 230℃.The internal standard was n-eicosane. This method had a recovery of 99.2%-100.9% ,the coefficient of variation was 0.262%. The operating conditions of high phase liquid chromatographic method were C18 column （250 mm×4.6 mm）, v （methanol）：v （water）=85：15 as mobile phase, spectrum length UV-262 nm. The recovery of this method was 98.9%-99.4% and the coefficient of variation of this method was 0.505%.

关 键 词：粉唑醇 分析 GC HPLC 

分 类 号：O657.72[理学—分析化学] TS272.51[理学—化学]