乙肝疫苗联合CpG-ODN或单独应用对孕鼠及新生鼠的影响  

Effects of the CpG motifs with or without HBsAg vaccine on the pregnant mice and their neonates

作　　者：张金萍[1] 肖昕[2] 蒋建伟[3] 刘秀香[2] 

机构地区：[1]复旦大学儿科医院新生儿科复旦大学上海医学院儿科学系,上海300032 [2]暨南大学围产医学中心新生儿科 [3]暨南大学生化教研室

出　　处：《临床儿科杂志》2005年第11期807-809,812,共4页Journal of Clinical Pediatrics

摘　　要：目的探讨CpG_ODN作为免疫佐剂对乙肝疫苗诱导孕鼠及其新生鼠体液免疫功能影响,并观察注射CpG_ODN是否导致妊娠并发症发生。方法用CpG1826联合乙型肝炎病毒疫苗免疫孕期BALB/c小鼠,ELISA法检测孕鼠及其新生鼠血清IgG抗体。结果CpG_ODN可明显提高正常成年鼠IgG抗体水平(P<0.05),对孕鼠及其子鼠血清IgG水平无影响(P>0.05);20μg/只的CpG1826剂量未影响新生鼠体重以及胎脑重量。结论以20μg/只的CpG1826剂量免疫孕鼠,孕鼠及其新生鼠没有出现免疫增强作用,也未导致胎儿宫内发育迟缓(1UGR)等妊娠并发症。CpG_ODN是一种新型免疫佐剂,有较大的开发价值及广泛的临床应用前景,但在妊娠群体应用尚待进一步研究。Objective To observe the influence of CpG-ODN on immune function of pregnant mice and their newborns and investigate if CpG-ODN injection can cause pregnant complications. Methods Serum antibody （IgG） levels in pregnant mice and neonatal mice were determined by ELISA after pregnant mice were immunized by CPG1826. Results CPG1826 increased serum IgG level in non-pregnant adult mice（95.89 ± 14.12μg/ ml vs 15.71 ± 5.64μg/ml） but did not influence the IgG level in the pregnant mice （10.97 ± 2.14μg/ml vs 9.28 ± 3.29 μg/ ml） and their neonates （1. 098± 0.32μg/ ml vs 0.856± 0.280μg/ ml） . With the dose of 20μg/ mouse, no side-effects such as intrauterine growth retardation（IUGR） occurred in the neonates. There was no difference in birth weight （1. 471 ± 0.11 g vs 1. 489± 0.15 g） between the neonates whose mother received CPG1826 or not. Conclusions With the dose of 20μg/mouse,CpG1826 do not produce any effects of immunological enhancement and any pregnant complications such as IUGR in pregnant mice and their neonates. CpG-ODN asa new immunity adjuvant has large development value and widely clinical application future.

关 键 词：CPG-ODN 免疫 孕鼠 新生鼠 

分 类 号：R714.7[医药卫生—妇产科学]