联合化疗诱导小鼠在体肿瘤多药耐药模型的建立  

Establishing Mice Model of Multidurg Resistance of Tumor Induced by Combined-chemotherapy

作　　者：徐龙进[1] 王祖梅[2] 孙付军[3] 李贵海[3] 李志彦[4] 李慧[1] 唐慧[1] 

机构地区：[1]山东省疾病预防控制中心,济南250014 [2]山东中医药大学附属医院,济南250011 [3]山东省中医药研究院,济南250014 [4]山东省实验动物中心,济南250015

出　　处：《实验动物与比较医学》2005年第4期215-217,共3页Laboratory Animal and Comparative Medicine

基　　金：山东省自然基金资助项目(编号:Y2002C37)

摘　　要：目的建立小鼠在体肿瘤多药耐药模型。方法模拟临床以亚于治疗剂量的联合化疗PFC 方案,诱导S180腹水型小鼠4周,流式细胞仪荧光检测P170、LRP、TOPOII,建立能客观反应临床肿瘤多药耐药产生机制并适合中药干预肿瘤多药耐药研究的在体细胞模型。结果化疗诱导后昆明小鼠S180腹水肿瘤细胞P170、LRP的表达率和ROPOII活性明显提高,经传代后其表达稳定。结论联合化疗诱导建立小鼠在体肿瘤多药耐药模型是可行的。Objective To establish the mice model ofmultidurg resistance of tumorous expression in ascites with S 180 cell. Methods The mice with S 180 cell in ascites were treated with the method of PFC of repeated-combined-chemotherapy in less than the therapy dose for 4 weeks. The levels of P170 and LRP and TOPOⅡ were detected by Flow Cytometry Analysis. Results The cellular multidurg rdsistance of tumorous expression （P170 and LRP） and the activity of TOPOⅡ of mice were improved obviously and the expression was stable in the cell line.

关 键 词：肿瘤 多药耐药 小鼠 P170 LRP TOPO Ⅱ 

分 类 号：R-332[医药卫生]