严重创伤多器官功能不全综合征大鼠模型建立  

Establishment of Rat Model of Multiple Organ Dysfunction Syndrome Caused by Trauma

作　　者：王占科[1] 杨莉萍[1] 褚芳 张玉珍[3] 刘龙燕[3] 余文[1] 胡扬涛[1] 

机构地区：[1]解放军第九四医院,南昌330002 [2]江西省实验动物学会,南昌330006 [3]江西医学院,南昌33000

出　　处：《实验动物与比较医学》2005年第4期218-220,232,共4页Laboratory Animal and Comparative Medicine

摘　　要：目的建立一种接近人类特征的严重创伤多器官功能不全综合征(MODS)动物模型。方法 60只SD大鼠随机分为创伤MODS模型组及正常对照组,雌雄各半,每组30只。创伤MODS模型组大鼠30％体表面积III度烫伤6 h后,腹腔注射大剂量内毒素(LPS)。正常对照组除不进行烫伤和腹腔注射内毒素外,其他处理方式与创伤MODS模型组相同。观察创伤MODS模型组及正常对照组伤后各时相点死亡例数和7 d内死亡率、以及伤后各时相点MODS相关指标[血丙氨酸氨基转移酶(ALT),肌酸激酶(CK),尿素氮(BUN),空腹血糖(FGLU)等]和促炎症细胞因子(血TNFα,NO和IL-6)水平动态变化。结果创伤MODS模型组大鼠死亡主要发生在烫伤1 d后,7 d内死亡率为53．33％,烫伤1 d后死亡占总死亡例数的87．5％,正常对照组无死亡。创伤 MODS模型组伤后各时相点血ALT,CK,BUN,FGLU等MODS相关指标以及血TNFα,NO和 IL-6水平均明显高于正常对照组(P<0．0001)。结论严重创伤后腹腔注射大剂量内毒素,可成功复制创伤MODS动物模型。Objective To establish the rat model of Multiple Organ Dysfunction Syndrome （MODS） caused by trauma. Method Sixty SD rats were divided randomly to MODS model group and control group. The rats of the MODS model group were scalded at 30% TBSA Ⅲ degree and injected with large amount of LPS 6 hour later. The mortality in 7 days, the blood ALT, CK,BUN, fasting glucose and TNFα, NO, IL-6 of MODS rats were observed or determined. Results The mortality in 7 days of the MODS rats was 53.33%. The blood ALT, CK, BUN, fasting glucose and blood TNFα, NO, IL-6 of the MODS rats were higher than that of the control. Conclusion Serious scalded rats with large amount of LPS injected can represents a characters of MODS.

关 键 词：大鼠 烫伤 内毒素 多器官功能不全综合征 

分 类 号：R-332[医药卫生]