微波辐射大鼠脏器组织超快制备电镜样品的实验研究  

Rapid Samples Preparation of Tissues from Rats for Electron Microscope Observation by Microwave Processing

作　　者：王响英[1] 吴淑燕[1] 李苏安[1] 毛棣华[1] 

机构地区：[1]苏州大学医学院基础医学系电镜室,苏州215007

出　　处：《实验动物与比较医学》2005年第4期221-225,共5页Laboratory Animal and Comparative Medicine

摘　　要：目的用大鼠脏器组织探索微波辐射透射电镜生物样品的超快制样方法,寻找适合本实验室条件的微波照射使用范围,以适应临床诊断工作的需要。方法取大鼠的肝,肾,心,肺,脾, 胰等脏器,按微波照射时间和功率不同分为微波1组、2组、3组,对样品进行快速制备,观察微波辐射后各组细胞器超微结构的保存结果,以选择最佳微波照射使用范围。结果三个微波组各脏器细胞超微结构在原位都能很好保存,超微结构清晰,境界分明,其中以微波2组效果最佳, 各脏器细胞超微结构与常规组无明显差异;微波1组肝和心脏线粒体有轻度膨胀外,其它脏器细胞超微结构完好;微波3组肝、肾、心脏器的部分细胞器略显微波损伤。结论微波照射使电镜生物样品制备时间大大缩短,由原来一周缩短到一天,并能良好地保持组织细胞的超微结构,得到清晰的图像,该微波仪可使用微波范围较广,重复性好,应用于电镜标本快速制备是可行的,缩短了临床病理电镜诊断的时间。Objective To satisfy the need of clinic diagnosis, a rapid samples preparation method of rat tissues for TEM observation by microwave process is investigated. Methods The samples of from viscera were divided into three groups numbered M1, M2 and M3 according to the difference in processing time and microwave power. Results The ultrastructures of the cells can be preserved intactly as original state and a clear ultrastructure and boundary can be observed in all groups. For M2 group, no evident ultrastructural difference can be seen between processed samples and normal samples. But for other groups, slight swelling of mitochondrion from liver and heart in M1 group and slight scathe from organoid of liver, kidney and heart in group M3 were found. Conclusion The preparation time of electron microscope samples can be greatly shorteneed by microwave process on the premise of intactly preserving ultrastructures of viscera cells, therefore shorten the diagnosis time in clinic.

关 键 词：微波幅射 大鼠 制备 电镜 样品 

分 类 号：R361[医药卫生—病理学]