比较以凋亡肿瘤细胞和肿瘤细胞裂解物制备疫苗的效果  

The research of tumor vaccine with apoptotic tumor cell and tumor cell lysate

作　　者：苏延军[1] 任秀宝[1] 王长利[1] 李慧[1] 郝希山[1] 

机构地区：[1]天津医科大学附属肿瘤医院,300060

出　　处：《中华微生物学和免疫学杂志》2005年第12期1002-1005,共4页Chinese Journal of Microbiology and Immunology

摘　　要：目的在肿瘤DC疫苗的研究当中,探讨最佳的抗原负载DC细胞的方式。方法采用137Cs照射处理后凋亡的肿瘤细胞、丝裂霉素(MMC)处理的凋亡肿瘤细胞、反复冻融肿瘤细胞的裂解物3种方法制备肿瘤抗原后负载DC细胞,再分别诱导T淋巴细胞。采用胞内染色法检测T细胞活化情况,LDH法检测T细胞的杀伤活性。结果照射、MMC诱导细胞凋亡以及裂解细胞提取蛋白3种方法制备的肿瘤抗原,分别负载DC后诱导T细胞,T细胞对特异性靶细胞的杀伤活性分别为47.31±4.89、41.65±4.08、38.91±3.55,照射组高于MMC组及裂解组(P<0.05),其余各组间差异无统计学意义(P>0.05);3组CD4+/IFN-γ+分别为1.85±0.37、1.37±0.37、1.24±0.32(n=5,P=0.044),照射组和MMC组明显高于裂解组,而照射组与MMC组差异无统计学意义(P=0.072)。CD8+/IFN-γ+分别为1.42±0.23、1.13±0.19、1.08±0.19(n=5,P=0.043),照射组明显高于裂解组(P=0.0001),其余各组间差异无统计学意义(P>0.05)。结论本研究显示凋亡的肿瘤细胞产生的杀伤能力以及T细胞分泌IFN-γ水平要高于肿瘤细胞裂解物,其中137Cs照射处理方法经比较为较佳的肿瘤疫苗形式。Objective To find apoptotic tumor cell and tumor cell lysate as antigens, presented by DC, and to induce special CTL in vitro. Methods Mature DC loading with apoptotic tumor cell and tumor cell lysate to stimulate autologous T cells in order to induce special CTL and to detect special lysis of corresponding target cells by LDH. Results ^137 Cs and MMC can induce tumor cell apoptosis with percentage as 93.0% ± 6.20%, 88.70% ± 8.28% separately. Apoptotic tumor cell induced by ^137 Cs or MMC and tumor cell lysate were used as tumor antigen to stimulate autologous T cells. The percentage of CD4^＋/WN-γ^＋ , CD8^＋/WN-γ^＋ and cytotoxicity of T cells stimulated by apoptotic tumor cells increased as compared with tumor lysate. Conclusion Apoptotic tumor cell can induce special CTL and potentially function the candidate of tumor vaccine.

关 键 词：肿瘤 DC疫苗 照射 裂解 丝裂霉素 

分 类 号：R730.3[医药卫生—肿瘤] R378.22[医药卫生—临床医学]