流式细胞术定量检测食管癌组织中P53、cyclin D1和Rb蛋白表达的意义  

Flow Cytometric Detection and Significance of P53,Cyclin D1 and Rb Proteins in Esopha-geal Cancer

作　　者：丁卫军[1] 刘志辉[2] 常建华[1] 黄朝晖[3] 李莉华[3] 

机构地区：[1]苏州大学附属第四医院心胸外科,无锡214062 [2]苏州大学附属第四医院 肿瘤研究所,无锡214062 [3]苏州大学附属第四医院肿瘤研究所,无锡214062

出　　处：《中国临床医学》2005年第6期1023-1024,共2页Chinese Journal of Clinical Medicine

摘　　要：目的:研究P53、cyclin D1和Rb蛋白在食管癌形成与发展中的作用。方法:应用流式细胞术对59例食管癌组织的cyclin D1、 P53和Rb蛋白进行定量检测。结果:cyclin D1、P53在低分化组的表达显著高于分化组(P<0．05);Rb在低分化组的表达低于分化组,但无显著差异(P>0．05);3种蛋白的表达与淋巴结转移均无相关性(P>0．05)。结论:P53和cyclin D1的表达与食管癌的分化密切相关;cyclin D1上调,P53失活可能是食管癌形成与发展的重要分子生物学机制。Objective： To explore the role of tumor suppressor gene P53 and cell-cycle related gene cyclin D1, Rb in the carcinogenesis and progression of esophageal cancer. Methods： The expression of P53, cyclin D1 and Rb in 59 esophageal cancer tissue species were detected by flow cytometry （FCM）. Results： The expressions of cyclin D1 and P53 in poorly-differentiated esophageal cancer tissue were lower than those in well-differentiated esophageal cancer tissue（P〈0. 05） ; The expression of Rb in poorly-differentiated esophageal cancer tissue was lower than those in well-differentiated esophageal cancer tissue, but no significant difference was found（P〉0. 05）. The expressions of all three proteins were not correlated with lymph node metastasis. Conclusion： The expressions of cyclin D1 and P53 are closely related to tumor differentiation of esophageal cancer;The up-regulation of cyclin D1 and deactivation of P53 may involved in the carcinogenesis and progression of esophageal cancer.

关 键 词：细胞周期调节蛋白D1 P53 RB 流式细胞术 食管癌 

分 类 号：R735.1[医药卫生—肿瘤]