槐耳浸膏诱导肝癌细胞凋亡的实验研究  

Research on Apoptosis of Human Hepetocellular Carcinoma Cells Induced by Trametes Rob-iniophila

作　　者：李立新[1] 叶胜龙[1] 王艳红[1] 孙瑞霞[1] 薛琼[1] 陈洁[1] 高东梅[1] 汤钊猷[1] 

机构地区：[1]复旦大学肝癌研究所中山医院,上海市肝脏肿瘤临床医学中心,上海200032

出　　处：《中国临床医学》2005年第6期1152-1153,共2页Chinese Journal of Clinical Medicine

基　　金：本课题受以下基金项目资助:复旦大学"211"工程肿瘤学科建设项目(校批字[2003]257号);上海市临床医学中心建设项目

摘　　要：目的:探索槐耳浸膏抑制肝癌细胞增殖的机理,为槐耳浸膏在临床上治疗肝肿瘤提供理论依据。方法:将MHCC97H肝癌细胞株分别与DMEM,槐耳浸膏(1 mg／ml,3 mg／ml,5 mg／ml,10 mg／ml)及5-FU(10μg／ml)作用24 h、48 h、72 h,在流式细胞仪上分别测定其细胞凋亡率。结果:不同浓度槐耳浸膏组在每一时段上诱导肝癌细胞凋亡率明显高于对照组。其凋亡率的升高有明显的剂量相关性。槐耳浸膏高浓度10mg／ml组的凋亡率与5-FU组相当。槐耳浸膏诱导肝癌细胞凋亡的作用随时间的延长而增加。结论: 槐耳浸膏能明显诱导肝癌细胞的凋亡,从而抑制肝癌细胞的增殖,为临床治疗肝肿瘤提供了依据。Objective： We explore the mechanism that Trametes robiniophila inhibits proliferation of the human hepetocellular carcinoma cells and provide the experimental basis for Trametes robiniophila treating liver cancer in clinic. Methods： The human hepetocellular carcinoma cells（MHCC97H）were treated with DMEM,Trametes robiniophila（1mg/ml,3mg/ml,5mg/ml,10mg/ml） and 5-Fu（10ug/ml） for 24h, 48h,72h respectively,and then the rates of apoptosis of the cells were measured in FACSCAlibur. Results： The rates of apoptosis of human hepetocellular carcinoma cells in different concentration groups of Trametes robiniophila in same time were higher than that of the control group, there is evident dose dependence in the rise of the rates of apoptosis in every Trametes robiniophila groups,but only the rate of apoptosis in 10mg/ml group corresponds to that of the 5-Fu group. The efficiency that Trametes robiniophila induces apoptosis in the human hepetocellular carcinoma cells amplify with time prolonging. Conclusion： Trametes robiniophila can apparently induce apoptosis in human hepetocellular carcinoma cells, thereby it can inhibit the proliferation of the cells.

关 键 词：槐耳浸膏 诱导 肝癌细胞 凋亡 

分 类 号：R735.7[医药卫生—肿瘤]