Heparanase-1和Syndecan-1与肝细胞癌侵袭转移的关系  

Relation of heparanase-1 and syndecan-1 gene to invasion and metastasis of hepatocellular carcinoma

作　　者：杨洁泉[1] 杨连粤[1] 彭吉祥[1] 王伟[1] 

机构地区：[1]中南大学湘雅医院肝癌研究室,长沙市410008

出　　处：《中华肝胆外科杂志》2005年第12期828-831,共4页Chinese Journal of Hepatobiliary Surgery

基　　金：国家"十五"科技攻关项目(2001BA703B04);国家自然科学基金资助项目(30371595)

摘　　要：目的探讨heparanase-1(HPR-1)和syndecan-1在孤立性大肝癌、结节性肝癌和小肝癌中的表达及其临床意义。方法选用30例肝细胞癌(HCC)癌组织和癌旁组织标本,用逆转录聚合酶链反应(RT-PCR)检测标本中的HPR-1和Syndecan-1的表达,并结合临床病理资料进行分析。结果HPR-1mRNA在HCC中的表达较癌旁肝组织高,差异有显著性意义(P=0·037);Syndecan-1在HCC中的表达较癌旁组织低,差异有显著性意义(P=0·007)。本研究显示HPR-1的高表达和Syndecan-1的低表达同有无包膜、静脉浸润密切相关,而同性别、细胞分化程度、肝硬化程度无关。将30例HCC分为孤立性大肝癌组,结节性肝癌组和小肝癌组分析发现:孤立性大肝癌组同结节性肝癌组HPR-1mRNA的表达有着显著性差异(P<0·05)。结论HPR-1基因和Syndecan-1基因与HCC的发生发展和侵袭转移密切相关;孤立性大肝癌较结节性肝癌具有较好的生物学行为。Objective To investigate the expression of heparanase-1 and syndecan-1 gene in solitary large hepatocellular carcinoma （HCC）, nodular HCC and small HCC and explore its clinical significance. Methods The mRNA expression of heparanase-1 and syndecan-1 was determined by RTPCR in samples of HCC and adjacent non cancerous tissue from 30 patients with HCC. Results The mRNA expression of heparanase-1 was significantly higher while that of syndecan-1 lower in tumor tissue than in the adjacent non-cancerous tissue （P〈0.05）. The high expression of heparanase-1 and low expression of syndecan 1 were correlated to capsular and venous invasion of HCC （P〈0.05）. The mRNA expression level of heparanase-1 was markedly higher in nodular HCC than in solitary large HCC and small HCC （P〈0.05）. Conclusions The heparanase-1 and syndecan-1 are correlated with the growth, invasion and metastasis of HCC. Solitary large HCC has better biological behavior than nodular HCC.

关 键 词：癌 肝细胞 Heparanase-1 SYNDECAN-1 基因表达 

分 类 号：R735.7[医药卫生—肿瘤]