HSP70、Ki-67、nm23-H1蛋白在鼻咽癌组织中表达  

Expression of HSP70,Ki-67 and nm23-H1 in na-sopharyngeal carcinoma

作　　者：黄瑾[1] 谈敏[2] 朱愉恒[1] 鲁长青[2] 谈炎[2] 

机构地区：[1]江苏省常州市第一人民医院放疗科,江苏常州213003 [2]江苏省常州市第一人民医院病理科,江苏常州213003

出　　处：《中国耳鼻咽喉头颈外科》2005年第12期756-760,共5页Chinese Archives of Otolaryngology-Head and Neck Surgery

摘　　要：目的探讨鼻咽癌组织中HSP70、Ki-67和nm23-H1蛋白表达的临床价值。方法采用免疫组织化学二步法,检测50例鼻咽癌患者鼻咽部或颈淋巴结组织中HSP70、Ki-67和nm23-H1蛋白表达水平。结果50例鼻咽癌组织中HSP70、Ki-67和nm23-H1阳性表达率分别为36.0%(18/50)、98.0%(49/50)和50.0%(25/50);HSP70和nm23-H1蛋白在Ⅱ-Ⅲ期和Ⅳa期中阳性表达率均有显著性差异(P=0.016,P=0.037);Binarylogistic回归模型分析HSP70、nm23-H1表达是鼻咽癌局部复发或远处转移的相关因素(P=0.022,P=0.038);Cox多因素回归模型分析HSP70表达是影响患者生存期的独立因素(P=0.001);nm23-H1与HSP70表达呈负相关(P=0.003);Ki-67表达与鼻咽癌局部复发或远处转移及生存期均无相关性。结论检测鼻咽癌组织中HSP70和nm23-H1表达对指导治疗及评价预后有重要的临床意义。OBJECTIVE To explore the clinical value of expression of HSP70, Ki-67 and nm23-H1 in nasopharyngeal carcinoma （NPC）. METHODS Expression of HSP70,Ki-67 and nm23-H1 were examined in primary tumor tissues or metastasis cervical lymph nodes of 50 patients with NPC by immunohistochemical techniques. RESULTS The positive rates of HSP70, Ki-67 and nm23-H1 in 50 patients were 36.0 %（18/50）, 98.0 %（49150） and 50.0 %（25/50） respectively. The positive expression of HSP70, nm23-H1 in Ⅱ -Ⅲ stages were significantly different from that of N a stage （P= 0.016,P = 0.037）. Expression of HSP70, nm23-H1 were the factor of regional recurrence or distant metastases in Binary logistic regression analysis （P =0.022,P=0.038）. COX multivariate analysis identified that expression of HSP70 was independent factor to overall survival （P=0.001）; Expression of nm23- H1 protein had negative correlation with HSP70（P =0.003）. Expression of Ki-67 had no correlation with regional recurrence or distant metastasis and overall survival. CONCLUSION The detection of HSP70, nm23-H1 expressions is valuable to the estimation of prognosis and treatment of NPC.

关 键 词：鼻咽肿瘤 热休克蛋白70 KI-67抗原 预后 

分 类 号：R739.63[医药卫生—肿瘤]