DSS诱导的急性实验性肠炎免疫细胞内GSH/GSSG与炎症损伤及细胞因子的关系  

Relationship bewteen GSH/GSSG with mucosal injury and cytokine profile in dextran sodium sulphate-induced acute colitis of mice

作　　者：张静[1] 韩英[1] 纪欣[1] 李虹义[1] 王志红[1] 

机构地区：[1]北京军区总医院消化内科,北京100700

出　　处：《中国医学工程》2005年第6期561-566,共6页China Medical Engineering

基　　金：北京市自然科学基金项目;编号7042064;项目名称:巨噬细胞调控在炎性肠病黏膜免疫异常中的作用

摘　　要：目的探讨硫酸葡聚糖钠(dextransodiumsulphate,DSS)诱导的小鼠肠炎模型腹腔巨噬细胞和脾细胞中还原型谷胱甘肽(GSH),氧化型谷胱甘肽(GSSG)的变化特征及其与Th1/Th2型细胞因子IFN-γ,IL-4的分泌及黏膜GSH表达与炎症损伤的关系。方法实验组小鼠给予含5%DSS的蒸馏水自由饮用7天之后处死,采集腹腔巨噬细胞及脾细胞,检测腹腔巨噬细胞及脾细胞中GSH与GSSG的变化。分离出的病变结肠一部分评价其病理学,另一部分结肠及脾脏培养后检测其IL-4、IFN-g的表达。结果DSS诱导的实验性肠炎小鼠腹腔巨噬细胞中的GSH较对照组降低,而GSSG较对照组增多,且GSH/GSSG比值明显减低。其脾细胞内GSH较对照组明显降低,GSH/GSSG比值较对照组降低但差异不明显。DSS诱导的实验性肠炎组病变结肠的IL-4明显升高,而其脾脏分泌的IFN-γ明显降低。结论DSS实验性肠炎中腹腔巨噬细胞是以氧化型巨噬细胞表形为主的。腹腔巨噬细胞及脾细胞中GSH的消耗、GSH/GSSG比值的减低与黏膜损坏及IL-4分泌增多、IFN-γ分泌减少相关。[Objective] To explore the expression of GSH/GSSG within celiac macrophages and splenic ceils in DSS-induced acute colitis and the relationship between GSH transformation and Th1/Th2 cytokine profile- IL-4 and IFN-g as well as mucosal injury. [Methods] BALB/C mice were fed with 5% DSS for 7 days to induce experimental colitis, and macrophages in abdominal cavity and splenic cells were collected, the GSH, GSSG and GSH/GSSG in celiac macrophages and in splenic cells were determined by Glutathione Assay Kits. The colon was isolated for both histopathological study and evaluation of IL-4 and IFN-g as well as GSH expression. [Results] The expression of GSH in celiac macrophages was downregulated but GSSG upregulated in DSS group, therefore the ratio of GSH/ GSSG decreased significantly compared with that of control group. The level of GSH in splenic cells reduced and the ratio of GSH/GSSG decreased but has no significance. A notable increase of IL-4 in colon and a decrease of IFN-g in spleen was noticed in the DSS-induced colitis. [Conclusion] The type of celiac macrophages in DSS-induced colitis is mainly oxidative macrophages （OMp）. Depletion of GSH and decreased ratio of GSH/GSSG in macrophages and splenic cells have a relationship with mucosal injury and high IL-4/low IFN-g cytokine profile.

关 键 词：硫酸葡聚糖钠 实验性肠炎 巨噬细胞 GSH/GSSG 

分 类 号：R574[医药卫生—消化系统]