TNF-α和IFN-γ对OSF成纤维细胞超微结构的影响  

Effects of tumor necrosis factor-α and interferon-γ on ultrastructure of OSF-derived fibroblasts

作　　者：吴颖芳[1] 彭解英[1] 许春姣[1] 唐瞻贵[1] 

机构地区：[1]中南大学湘雅医院口腔科,湖南长沙410008

出　　处：《中国医学工程》2005年第6期594-596,599,共4页China Medical Engineering

基　　金：湖南省科委发展基金资助(99ssy2002-14)

摘　　要：目的研究TNF-α、IFN-γ对正常人颊黏膜成纤维细胞(NM-FB)和口腔黏膜下纤维化(Oralsubmucousfibrosis,OSF)患者颊黏膜成纤维细胞(OSF-FB)超微结构的改变。方法取6例OSF患者及5例正常人颊黏膜组织,采用组织块法进行成纤维细胞原代培养并传代,电镜下观察不同浓度TNF-α及IFN-γ对NM-FB和OSFF-FB超微结构的改变。结果网池扩张,线粒体旺盛。而IFN-γ处理后上述细胞器减少,线粒体减少并肿胀。结论TNF-α促进NM-FB和OSF-FB的胶原合成,而IFN-γ对两者的胶原合成具有抑制作用。[Objection] To investigate the effects of tumor necrosis factor-α（TNF-α） and interferon-γ （IFN-γ） on the ultrastructure of fibroblasts from normal persons（NM-FB） and patients with oral submucous fibrosis （OSF- FB）. [Methods] Fibroblast cultures were established from the buccal mucosa of five normal person and six patients with OSF, The study focused on the effects of TNF-α and IFN-γ on the ultrastructure of NM-FB and OSF-FB by electronmicroscopy. [Results] The ultrastructure of NM-FB and OSF-FB were changed correspondingly by the two cytokines treatment. [Conclusions] The authors believe that TNF-α can upregulate and IFN-γ can deregulate the progression of OSF.

关 键 词：口腔黏膜下纤维化 成纤维细胞 超微结构 TNF—α IFN-Γ 

分 类 号：R781.5[医药卫生—口腔医学]