微生物薯蓣皂苷糖苷酶的分离纯化的研究  

Purification of Dioscinase from microorganism

作　　者：刘冰[1] 王元好[1] 胡湘春[1] 鱼红闪[1] 金凤燮[1] 

机构地区：[1]大连轻工业学院生物与食品工程学院,辽宁大连116001

出　　处：《微生物学杂志》2005年第6期6-9,共4页Journal of Microbiology

基　　金：国家自然科学基金项目(30371744;20476017;30470055)

摘　　要：产薯蓣皂苷酶的sp.s00c菌发酵液,通过分级沉淀的分离方法,经离子交换柱提纯分离,得到聚丙烯酰胺凝胶电泳单点的酶,其酶蛋白分子量为59 ku,提纯酶最适酶反应温度为40℃,最适pH值为5.0。提纯酶不仅能水解薯蓣皂苷的鼠李糖基,也能水解薯蓣皂苷的葡萄糖基。The dioscin-glycosidase from microorganism were purified from sp. s00c by the separation method that gradings deposit and ion-change resin column to obtain one spot in the SDS-polyacrylamide gel eletrophoresis. The enzyme molecular weight was 59 ku. The enzyme optimum reaction condition was in pH5.0 and at 40℃ for reaction 12 to 24h; the purified enzyme can hydrolyze both the rhamnoside and glucoside of dioscin which differentiate.

关 键 词：薯蓣皂苷 糖苷酶 纯化 分子量 

分 类 号：Q946.83[生物学—植物学]