黄芪当归合剂对大鼠肾间质纤维化的影响及机制探讨  

Effect of astragalus angelica mixture on rats models with renal interstitial fibrosis and mechanism

作　　者：颜润[1] 俞雷[1] 张义雄[1] 龙丽[1] 

机构地区：[1]贵阳医学院附属医院肾内科,550004

出　　处：《贵州医药》2006年第1期8-10,共3页Guizhou Medical Journal

摘　　要：目的探讨黄芪当归合剂(A&A)对肾间质纤维化模型大鼠的影响并探讨其可能的作用机制。方法采用单侧输尿管结扎(UUO)致大鼠肾间质纤维化模型。将大鼠随机分为3组:假手术组、模型组、黄芪当归合剂治疗组。手术后第9d处死各组大鼠,分别经免疫组化方法观察各组大鼠肾组织的血小板源性生长因子-B(PDGF-B)的表达部位、表达水平及α-平滑肌肌动蛋白(α-SMA)、增殖细胞核抗原(PCNA)的表达情况。Masson染色评定各组肾小管间质损害程度。结果模型组 PDGF-B、α-SMA、PCNA的表达及肾小管间质损伤指数明显高于假手术组(P<0．05),而黄芪当归合剂组均明显低于模型组(P<0．05)。各项指标作相关分析PDGF-B与肾小管间质损伤指数(r= 0．870,P<0．01)、α-SMA(r=0．956,P<0．01)、PCNA(r=0.881,P<0.01)呈显著正相关。结论黄芪当归合剂可能通过下调PDGF-B的表达减轻肾小管间质纤维化。Objective To investigate the effect and the mechanism of astragalus angelica mixture （A＆A） on rat models with renal interstitial fibrosis： Methods Nine days after unilateral ureteral obstruction （UUO） of animal models were used. Expression of PDGF-B, α-SMA and PCNA of the obstructed kidneys were assessed by immunohistochemistry and the degree of tubulointerstitial damage was scored. Results The levels of platelet- derived growth factor- b （PDGF-B）, α-SMA and PCNA in the model group were increased remarkably compared with the sham- operated group （P〈0. 05）, and paralleled with the degree of tubulointerstitial damage. The levels of PDGF-B, α-SMA and PCNA were significantly reduced by A＆A treatment. PDGF-B has a positive correlation with the index of tubulointerstitial damage, α-SMA and PCNA. Conclusion A＆A may prevent fibrosis development via down regulation the expression of PDGF-B partly.

关 键 词：黄芪 当归 肾间质纤维化 血小板源性生长因子-B 

分 类 号：R365[医药卫生—病理学]