人颊癌BCaCD885细胞加热后细胞膜HSP70的动力学变化与热耐受性的关系  

The relationship between thermotolerance and expression of membrane HSP70 in human buccal carcinoma BCaCD885 cells after heat treatment

作　　者：徐平平[1] 申晓青[2] 毛祖彝[3] 

机构地区：[1]广东省口腔医院,广州510260 [2]南方医科大学珠江医院,广州510280 [3]四川大学华西口腔医学院,成都610041

出　　处：《广东医学》2006年第1期28-30,共3页Guangdong Medical Journal

基　　金：广东省医学科学技术研究基金资助项目(编号:B1999017)

摘　　要：目的探讨肿瘤细胞热耐受性与细胞膜热休克蛋白表达的关系。方法采用流式细胞仪检测了人颊癌BCaCD885细胞经43.5℃加热40 min后2,4,6,8,12,24,48及72 h细胞膜热休克蛋白70的表达动力学,并与相隔不同时间接受2次43.5℃,40 min加热的BCaCD885细胞产生的热耐受性情况进行比较。结果加热后直至6 h时细胞膜热休克蛋白70的表达才有较明显的升高,约在12 h时达到高峰,尔后开始下降,到72 h时基本恢复到对照组的水平。细胞膜热休克蛋白70表达水平和细胞热耐受性的消长有一定的相关性,相关系数为0.840。结论热休克蛋白70在细胞热耐受性形成过程中具有较重要的作用,结果可为肿瘤临床热疗和免疫治疗提供参考。Objective To investigate the mechanism of thermotolerance. Methods The survival rate and dynamic expression of membrane heat shock protein 70 （HSP70） of a human buccal carcinoma cell line BCaCD885 were determined after heat treatment （HT） in 43.5℃ water bath for 40 minutes. Results Expression of membrane HSP70 did not increase until 6 hours after HT, reached peak value in 12 hours, and decreased gradually. The relative coefficient between thermotolerance and membrane HSP70 was 0. 840. Conclusion Membrane HSP70 may account for the development and decay of thermotolerance. The potential signifieance of the results is implied in thermotherapy and immunotherapy for tumors.

关 键 词：肿瘤 热疗 热休克蛋白70 热耐受性 

分 类 号：R739.8[医药卫生—肿瘤] R135.3[医药卫生—临床医学]